Cloned (Comment) | Organism |
---|---|
- |
Tequatrovirus T4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by pamidronate | Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
methylenebisphosphonate | in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C | Tequatrovirus T4 | |
0.08 | - |
clodronate | in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C | Tequatrovirus T4 | |
0.73 | - |
etidronate | in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C | Tequatrovirus T4 | |
1.3 | - |
tripolyphosphate | in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | T4 DNA ligase catalyzes the formation of phosphodiester bonds between neighboring 3'-hydroxyl and 5'-phosphate ends in doublestranded DNA | Tequatrovirus T4 | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
ATP + clodronate | - |
Tequatrovirus T4 | adenosine 5'-triphosphate derivative of clodronate + diphosphate | - |
? | |
ATP + etidronate | - |
Tequatrovirus T4 | adenosine 5'-triphosphate derivative of etidronate + diphosphate | - |
? | |
ATP + methylenebisphosphonate | - |
Tequatrovirus T4 | adenosine 5'-triphosphate derivative of methylenebisphosphonate + diphosphate | - |
? | |
ATP + tripolyphosphate | - |
Tequatrovirus T4 | adenosine 5'-triphosphate derivative of tripolyphosphate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
T4 DNA ligase | - |
Tequatrovirus T4 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
methylenebisphosphonate | in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C | Tequatrovirus T4 | |
0.0041 | - |
clodronate | in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C | Tequatrovirus T4 | |
0.0062 | - |
tripolyphosphate | in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C | Tequatrovirus T4 | |
0.07 | - |
etidronate | in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C | Tequatrovirus T4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Tequatrovirus T4 |