Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | 30 mM, more than 60% reduction in product formation | Plasmodium falciparum | |
NaCl | above 20 mM, more than 60% reduction in product formation | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000026 | - |
nicked DNA | 37°C, pH 8.5 | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enzyme can utilize Ca2+ as cofactor | Plasmodium falciparum | |
Mg2+ | maximal activation at 0.6 mM | Plasmodium falciparum | |
Mn2+ | maximal activation at 6 mM | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | PfLigI is able to join RNA-DNA substrates only when the RNA sequence is upstream of the nick. Slight activity with dATP. No activity with NAD+, UTP, CTP and GTP. Slight activity with dATP. No activity with NAD+, UTP, CTP or GTP | Plasmodium falciparum | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + nicked DNA | - |
Plasmodium falciparum | AMP + diphosphate + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA ligase I | - |
Plasmodium falciparum |
PfLigI | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Plasmodium falciparum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | about 50% of maximal activity at 25°C and at 40°C | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
nicked DNA | 37°C, pH 8.5 | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | pH 7.5: about 40% of maximal activity, pH 9.5: about 40% of maximal activity | Plasmodium falciparum |