KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
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additional information | - |
additional information | Km-values increase by 90-100-fold for 8-oxo-7,8-dihydroguanosine/C-, 8-oxo-7,8-dihydroinosine/C-, 8-oxo-7,8-dihydroinosine/A-, and 8-oxo-7,8-dihydroadenosine/T-containing duplexes compared to that of a G/C-containing duplex. Substrates containing guanidinohydantoin/A, guanidinohydantoin/G, spiroiminodihydantoin/A, and spiroiminodihydantoin/G base pairs exhibited Km values 20-70-fold higher than that of the substrate containing a G/base pair, while the Km value for 8-oxo-7,8-dihydroguanosine/A is 5 times lower than that for G/C | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
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Tequatrovirus T4 | - |
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Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | enzyme is most sensitive to lesions on the 3'end of the nick compared to the 5'end and to lesions located in the intact template strand. Substrates containing the 8-oxo-7,8-dihydroguanosine/A mismatch are more readily ligated than those with the 8-oxo-7,8-dihydroguanosine/C mismatch. Ligations of duplexes containing the 8-oxo-7,8-dihydroguanosine/T base pair that could adopt an anti-anti conformation proceeds with high efficiencies. An 8-oxo-7,8-dihydroguanosine/A mismatch-containing duplex behaves like 8-oxo-7,8-dihydroinosine/A. Km-values increase by 90-100-fold for 8-oxo-7,8-dihydroguanosine/C-, 8-oxo-7,8-dihydroinosine/C-, 8-oxo-7,8-dihydroinosine/A-, and 8-oxo-7,8-dihydroadenosine/T-containing duplexes compared to that of a G/C-containing duplex. Substrates containing guanidinohydantoin/A, guanidinohydantoin/G, spiroiminodihydantoin/A, and spiroiminodihydantoin/G base pairs exhibit Km values 20-70fold higher than that of the substrate containing a G/base pair, while the Km value for 8-oxo-7,8-dihydroguanosine/A is 5 times lower than that for G/C | Tequatrovirus T4 | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
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