Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | nick sealing by LigD in the presence of 0.1 mM ATP requires a divalent cation cofactor. Cobalt supports the conversion of the 12-mer pDNA strand to a 24-mer which es elongated to 25-mer and 26-mer products | Pseudomonas aeruginosa | |
Mg2+ | nick sealing by LigD in the presence of 0.1 mM ATP requires a divalent cation cofactor. Magnesium supports the conversion of the 12-mer pDNA strand to a discrete 24-mer ligation product. An additional minor species corresponding to AppDNA is also produced | Pseudomonas aeruginosa | |
Mn2+ | nick sealing by LigD in the presence of 0.1 mM ATP requires a divalent cation cofactor. Conversion of the 12-mer pDNA strand results in ligated products consisting of a triplet of 24-, 25-, and 26-mer species. In addition, manganese prompted the appearance of discrete radiolabeled 13- and 14-mer species | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | intrinsic polymerase function resident within an autonomous C-terminal polymerase domain, LigD-(533840), that flanks an autonomous DNA ligase domain, LigD-(188527) | Pseudomonas aeruginosa | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
LigD | - |
Pseudomonas aeruginosa |