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Literature summary for 6.5.1.1 extracted from

  • Cai, L.; Hu, C.; Shen, S.; Wang, W.; Huang, W.
    Characterization of bacteriophage T3 DNA ligase (2004), J. Biochem., 135, 397-403.
    View publication on PubMed
Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, polyhistidine-tagged enzyme Enterobacteria phage T3

Inhibitors

Inhibitors Comment Organism Structure
ATP 2 mM 50% inhibition Enterobacteria phage T3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0144
-
 ATP ligation of cohesive DNA Enterobacteria phage T3

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, increases activity 9fold Enterobacteria phage T3
Mg2+ 5 mM, increases activity 9fold Enterobacteria phage T3
Mn2+ 5 mM, increases activity 7fold Enterobacteria phage T3
NaCl promotes enzyme activity, optimal concentration: 300 mM Enterobacteria phage T3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Enterobacteria phage T3
-
 AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Enterobacteria phage T3
-
 dAMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Organism

Organism UniProt Comment Textmining
Enterobacteria phage T3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant polyhistidine-tagged enzyme Enterobacteria phage T3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Enterobacteria phage T3 AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Enterobacteria phage T3 dAMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
8
-
-
 Enterobacteria phage T3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Enterobacteria phage T3

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 pH 6.5: about 80% of maximal activity, pH 8.5: about 85% of maximal activity Enterobacteria phage T3

pI Value

Organism Comment pI Value Maximum pI Value
Enterobacteria phage T3 calculation from amino acid sequence
-
 5.14