Literature summary for 6.5.1.1 extracted from

Wu, Y.Q.; Hohn, B.; Ziemienowicz, A.
Characterization of an ATP-dependent type I DNA ligase from Arabidopsis thaliana (2001), Plant Mol. Biol., 46, 161-170.

Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression of intein-DNA ligase I fusion protein in Escherichia coli	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.003	-	ATP	37°C, pH 7.5	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
87000	-	x * 87000, SDS-PAGE	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	Arabidopsis thaliana	-	AMP + diphosphate + (deoxyribonucleotide)m+n	-	r

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant intein-DNA ligase I fusion protein, chitin beads, Affi Gel Blue	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Arabidopsis thaliana	AMP + diphosphate + (deoxyribonucleotide)m+n	-	r
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	DNA ligase I is able to ligate nicks in oligo(dT)/poly(dA) and oligo(rA)/poly(dT) substrates but not in oligo(dT)/poly(rA) substrates, double-stranded DNA's with cohesive or blunt ends are also good substrates	Arabidopsis thaliana	AMP + diphosphate + (deoxyribonucleotide)m+n	ability of DNA ligase I to revert the ligation reaction by relaxing the supercoiled DNA	r

Subunits

Subunits	Comment	Organism
?	x * 87000, SDS-PAGE	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
DNA ligase I	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)