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Literature summary for 6.5.1.1 extracted from

  • Magnet, S.; Blanchard, J.S.
    Mechanistic and kinetic study of the ATP-dependent DNA ligase of Neisseria meningitidis (2004), Biochemistry, 43, 710-717.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Neisseria meningitidis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000029
-
(deoxyribonucleotide)20 25°C, pH 7.5 Neisseria meningitidis
0.0004
-
ATP 25°C, pH 7.5 Neisseria meningitidis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ approx. 10% of activity with Mg2+ Neisseria meningitidis
Co2+ approx. 12% of activity with Mg2+ Neisseria meningitidis
Mg2+ absolutely required for activity, maximal activity at 2 mM Neisseria meningitidis
Mn2+ approx. 20% of activity with Mg2+ Neisseria meningitidis
Ni2+ approx. 18% of activity with Mg2+ Neisseria meningitidis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000 32000 analytical ultracentrifugation Neisseria meningitidis
31540
-
gel filtration after preincubation with Mg2+ and diphosphate Neisseria meningitidis
31548
-
1 * 31548, deduced from nucleotide sequence Neisseria meningitidis
31873
-
1 * 31873, mass spectroscopy Neisseria meningitidis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (deoxyribonucleotide)20 + (deoxyribonucleotide)20 Neisseria meningitidis sealing of a single nick in a 20mer DNA duplex, ADL is specific for nicked DNA and is not able to catalyze blunt end joining AMP + diphosphate + (deoxyribonucleotide)40
-
?

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant his-tagged ADL, nickel-affinity chromatography Neisseria meningitidis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)20 + (deoxyribonucleotide)20 sealing of a single nick in a 20mer DNA duplex, ADL is specific for nicked DNA and is not able to catalyze blunt end joining Neisseria meningitidis AMP + diphosphate + (deoxyribonucleotide)40
-
?

Subunits

Subunits Comment Organism
monomer 1 * 31548, deduced from nucleotide sequence Neisseria meningitidis
monomer 1 * 31873, mass spectroscopy Neisseria meningitidis

Synonyms

Synonyms Comment Organism
ADL
-
Neisseria meningitidis
ATP-dependent DNA ligase
-
Neisseria meningitidis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0018
-
(deoxyribonucleotide)20 25°C, pH 7.5 Neisseria meningitidis