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Literature summary for 6.5.1.1 extracted from

  • Matsuda, S.; Sakaguchi, K.; Tsukada, K.; Teraoka, H.
    Characterization of DNA ligase from the fungus Coprinus cinereus (1996), Eur. J. Biochem., 237, 691-697.
    View publication on PubMed
Search for more literature for 6.5.1.1

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate
-
 Coprinopsis cinerea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00009
-
 (dT)20 hybridized with (dA) Coprinopsis cinerea
0.0015
-
 ATP
-
 Coprinopsis cinerea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Coprinopsis cinerea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
 1 * 88000, SDS-PAGE Coprinopsis cinerea
90000
-
 gel filtration Coprinopsis cinerea

Organism

Organism UniProt Comment Textmining
Coprinopsis cinerea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Coprinopsis cinerea

Source Tissue

Source Tissue Comment Organism Textmining
mycelium mitotic cell Coprinopsis cinerea
-
pachytene cell
-
 Coprinopsis cinerea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Coprinopsis cinerea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m capable of joining (dT)20*(rA)n and (rA)12-18*(dT)n as well as (dT)20*(dA)n, ligation of blunt-ended DNA in the presence of polyethylene glycol 6000 Coprinopsis cinerea AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (dT)20
-
 Coprinopsis cinerea AMP + diphosphate + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 88000, SDS-PAGE Coprinopsis cinerea