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Literature summary for 6.5.1.1 extracted from

  • Teraoka, H.; Sawai, M.; Tsukada, K.
    Kinetic studies on the reaction catalyzed by DNA ligase from calf thymus (1983), Biochim. Biophys. Acta, 747, 117-122.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
spermidine 5 mM, increases the Km for nicked DNA, increases reaction velocity Bos taurus
spermine 0.5 mM, increases the Km for nicked DNA, increases reaction velocity Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
dATP competitive with respect to ATP Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00011
-
DNA nicked DNA 5'-phosphoryl ends Bos taurus
0.002
-
ATP ATP Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bos taurus
Mg2+ optimal concentration: 10 mM Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-

Reaction

Reaction Comment Organism Reaction ID
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate uni-uni uni-bi ping-pong mechanism. The order of substrate addition and product release is as follows: ATP, diphosphate, nicked DNA, sealed DNA, 5'-AMP Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Bos taurus AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
ATP + DNA
-
Bos taurus AMP + diphosphate + ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.117
-
DNA
-
Bos taurus