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Literature summary for 6.5.1.1 extracted from

  • Pedrali Noy, G.C.F.; Spadari, S; Ciarrocchi, G.; Pedrini, A.M.; Falaschi, A.
    Two forms of the DNA ligase of human cells (1973), Eur. J. Biochem., 39, 343-351.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens
Mg2+ Mn2+ and Ca2+ cannot replace Mg2+ in activation Homo sapiens
Mn2+ Mn2+ cannot replace Mg2+ in activation Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
DNA ligase II, gel filtration Homo sapiens
190000
-
and a lower MW form of MW 95000, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
EUE cell heteroploid Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol w/w, 3 mg/ml bovine serum albumin, stable for up to 7 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase can reconstitute the transforming activity of Bacillus subtilis DNA inactivated by pancreatic DNAse, unable to use a hybrid substrate where an interrupted polydeoxynucleotide is annealed to a polyribonucleotide Homo sapiens AMP + diphosphate + (deoxyribonucleotide)n+m
-
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