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Literature summary for 6.5.1.1 extracted from

  • Rabin, B.A.; Hawley, R.S.; Chase, J.W.
    DNA ligase from Drosophila melanogaster embryos. Purification and physical characterization (1986), J. Biol. Chem., 261, 10637-10645.
    View publication on PubMed
Search for more literature for 6.5.1.1

General Stability

General Stability Organism
retains 90% of its activity after 3 cycles of freeze-thawing Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
NEM
-
 Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79800
-
 gel filtration Drosophila melanogaster
83000
-
 1 * 83000, SDS-PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Drosophila melanogaster DNA ligase is biologically active to endonucleolytically cleaved pBR322 DNA ?
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
egg unfertilized, high activity of DNA ligase Drosophila melanogaster
-
embryo
-
 Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Drosophila melanogaster

Storage Stability

Storage Stability Organism
-80°C, stable for at least 12 months Drosophila melanogaster
0°C, stable for at least 6 months Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase is biologically active to endonucleolytically cleaved pBR322 DNA Drosophila melanogaster ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 83000, SDS-PAGE Drosophila melanogaster