Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Reducing agent | reducing agents, e.g. 2-mercaptoethanol or dithiothreitol required | Mammalia | |
Reducing agent | reducing agents, e.g. 2-mercaptoethanol or dithiothreitol required | eukaryota | |
Reducing agent | reducing agents, e.g. 2-mercaptoethanol or dithiothreitol required | Tequatrovirus T4 | |
Reducing agent | reducing agents, e.g. 2-mercaptoethanol or dithiothreitol required | Escherichia phage T7 |
Application | Comment | Organism |
---|---|---|
analysis | essential reagent in studies on nucleic acid structure and metabolism. In combination with polynucleotide kinase end-group labeling, DNA ligase can be used to identify 3'- and 5'-end groups at single-strand interruptions by nearest neighbor analysis. DNA | Mammalia |
analysis | essential reagent in studies on nucleic acid structure and metabolism. In combination with polynucleotide kinase end-group labeling, DNA ligase can be used to identify 3'- and 5'-end groups at single-strand interruptions by nearest neighbor analysis. DNA | Tequatrovirus T4 |
analysis | essential reagent in studies on nucleic acid structure and metabolism. In combination with polynucleotide kinase end-group labeling, DNA ligase can be used to identify 3'- and 5'-end groups at single-strand interruptions by nearest neighbor analysis. DNA | Escherichia phage T7 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cs+ | - |
Tequatrovirus T4 | |
dATP | - |
Escherichia phage T7 | |
dATP | - |
Mammalia | |
dATP | competitive with respect to ATP | Tequatrovirus T4 | |
K+ | - |
Tequatrovirus T4 | |
Li+ | - |
Tequatrovirus T4 | |
Na+ | - |
Tequatrovirus T4 | |
NH4+ | - |
Tequatrovirus T4 | |
spermidine | - |
Tequatrovirus T4 | |
spermine | - |
Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mammalia | |
0.0000015 | - |
DNA | internal phosphomonoesters in nicked natural DNA | Tequatrovirus T4 | |
0.0002 | 0.0015 | ATP | DNA ligase I | Mammalia | |
0.0003 | - |
ATP | ATP-diphosphate exchange reaction | Escherichia phage T7 | |
0.0006 | - |
DNA | for either the joining of oligo(dT)10 on poly(dA) or the joining of DNA fragments with a two base-pair overhang generated by a restriction enzyme | Tequatrovirus T4 | |
0.004 | - |
dATP | dATP-diphosphate exchange reaction | Escherichia phage T7 | |
0.006 | - |
ATP | joinig reaction | Escherichia phage T7 | |
0.014 | - |
ATP | joining reaction | Tequatrovirus T4 | |
0.045 | 0.1 | ATP | DNA ligase II | Mammalia | |
0.05 | - |
DNA | blunt end joining | Tequatrovirus T4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mammalia | |
Mg2+ | required | Tequatrovirus T4 | |
Mg2+ | optimal concentration: 10 mM | Tequatrovirus T4 | |
Mn2+ | 25% as effective as Mg2+ in activation | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
1 * 63000, PAGE under denaturing and reducing conditions | Tequatrovirus T4 |
68000 | - |
gel filtration | Tequatrovirus T4 |
85000 | - |
gel filtration | Mammalia |
200000 | - |
gel filtration | Mammalia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Mammalia | - |
? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Saccharomyces cerevisiae | - |
? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Tequatrovirus T4 | DNA ligase mutations drastically affect DNA synthesis, little effect on genetic recombination and repair of UV damage | ? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Escherichia phage T7 | mutants fail to produce progeny phage when grown on ligase-deficient strains of E. coli | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage T7 | - |
- |
- |
eukaryota | - |
- |
- |
Mammalia | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Schizosaccharomyces pombe | - |
- |
- |
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Mammalia | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
eukaryota | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Saccharomyces cerevisiae | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Schizosaccharomyces pombe | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | catalyzes blunt end joining of DNA | Tequatrovirus T4 | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA ligase joins oligo(dT)*poly(A) | Escherichia phage T7 | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | joins DNA annealed to RNA and, to a slight extent, even RNA annealed to its complementary RNA strand | Tequatrovirus T4 | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Mammalia | ? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Saccharomyces cerevisiae | ? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA ligase mutations drastically affect DNA synthesis, little effect on genetic recombination and repair of UV damage | Tequatrovirus T4 | ? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | mutants fail to produce progeny phage when grown on ligase-deficient strains of E. coli | Escherichia phage T7 | ? | - |
? | |
ATP + DNA | - |
Tequatrovirus T4 | AMP + diphosphate + ? | - |
? | |
dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Mammalia | dAMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | at 0.5% of the activity relative to ATP | Tequatrovirus T4 | dAMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | at 35-50% of the activity relative to ATP | Escherichia phage T7 | dAMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
additional information | - |
Mammalia | ? | - |
? | |
additional information | ATP-diphosphate exchange reaction | Tequatrovirus T4 | ? | - |
? | |
additional information | ATP-diphosphate exchange reaction | Escherichia phage T7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Mammalia |
monomer | - |
Saccharomyces cerevisiae |
monomer | - |
Escherichia phage T7 |
monomer | 1 * 63000, PAGE under denaturing and reducing conditions | Tequatrovirus T4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
joining of the blunt ends of duplex structures 16 nucleotides or longer | Tequatrovirus T4 |
25 | - |
blunt end ligation, 16mer or longer | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.8 | joining of nicks in Tris-HCl buffer | Tequatrovirus T4 |
7.2 | 7.7 | in Tris-HCl buffer | Escherichia phage T7 |
7.4 | 8 | DNA ligase I, in Tris-HCl buffer | Mammalia |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | 8 | 6.9: 46% of maximal activity, 8.0: 65% of maximal activity | Tequatrovirus T4 |
7.2 | 8.4 | 7.2-7.7: maximal activity, 8.4: 50% of maximal activity | Escherichia phage T7 |