Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
- |
Schizosaccharomyces pombe |
- |
Vaccinia virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
DNA | nicked DNA, enzyme DNA ligase II | Bos taurus | |
0.00011 | - |
DNA | nicked DNA, enzyme DNA ligase I | Bos taurus | |
0.001 | - |
ATP | - |
Drosophila melanogaster | |
0.002 | - |
ATP | DNA ligase I | Bos taurus | |
0.002 | - |
ATP | ATP, ATP-diphosphate exchange reaction | Bos taurus | |
0.04 | - |
ATP | DNA ligase II | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Drosophila melanogaster | 5634 | - |
nucleus | - |
Mammalia | 5634 | - |
nucleus | DNA ligase II is more firmly retained in isolated cell nuclei than DNA ligase I | Bos taurus | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
x * 85000, calculation from nucleotide sequence, SDS-PAGE | Saccharomyces cerevisiae |
86000 | - |
x * 86000, calculation from nucleotide sequence, SDS-PAGE | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Drosophila melanogaster | - |
DNA ligase I and II | - |
Mammalia | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Schizosaccharomyces pombe | - |
- |
- |
Vaccinia virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DNA ligase I and II | Mammalia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Drosophila melanogaster | - |
thymus | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | can join a 3'-hydroxyl terminus of DNA to a 5'-hydroxyl terminus of RNA, can join oligo(rA)12*poly(dT), blunt end joining in presence of polyethylene glycol | Drosophila melanogaster | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | inability to ligate oligo(dT)*poly(rA) | Drosophila melanogaster | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | inability to ligate oligo(dT)*poly(rA) | Mammalia | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | inability to ligate oligo(dT)*poly(rA) | Saccharomyces cerevisiae | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | inability to ligate oligo(dT)*poly(rA) | Bos taurus | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | inability to ligate oligo(dT)*poly(rA) | Schizosaccharomyces pombe | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | inability to ligate oligo(dT)*poly(rA) | Vaccinia virus | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + DNA | - |
Bos taurus | AMP + diphosphate + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Drosophila melanogaster |
? | x * 120000-130000, SDS-PAGE, gel filtration under denaturing conditions, DNA ligase I | Bos taurus |
? | x * 85000, calculation from nucleotide sequence, SDS-PAGE | Saccharomyces cerevisiae |
? | x * 86000, calculation from nucleotide sequence, SDS-PAGE | Schizosaccharomyces pombe |