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Literature summary for 6.4.1.7 extracted from

  • Aoshima, M.; Igarashi, Y.
    A novel oxalosuccinate-forming enzyme involved in the reductive carboxylation of 2-oxoglutarate in Hydrogenobacter thermophilus TK-6 (2006), Mol. Microbiol., 62, 748-759.
    View publication on PubMed
Search for more literature for 6.4.1.7

Cloned(Commentary)

Cloned (Comment) Organism
a fragment containing alpha and beta subunit genes is engineered for heterologous expression in Escherichia coli Hydrogenobacter thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.788
-
 ATP pH 8.5, 20°C Hydrogenobacter thermophilus
1.03
-
 2-oxoglutarate pH 8.5, 20°C Hydrogenobacter thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-oxoglutarate + HCO3- Hydrogenobacter thermophilus first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 ADP + phosphate + oxalosuccinate
-
 ?
ATP + 2-oxoglutarate + HCO3- Hydrogenobacter thermophilus TK-6 / IAM 12695 first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 ADP + phosphate + oxalosuccinate
-
 ?

Organism

Organism UniProt Comment Textmining
Hydrogenobacter thermophilus
-
 TK-6
-
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
 TK-6
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Hydrogenobacter thermophilus

Storage Stability

Storage Stability Organism
4°C, stable for at least 1 month Hydrogenobacter thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-oxoglutarate + HCO3-
-
 Hydrogenobacter thermophilus ADP + phosphate + oxalosuccinate
-
 ?
ATP + 2-oxoglutarate + HCO3- first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 Hydrogenobacter thermophilus ADP + phosphate + oxalosuccinate
-
 ?
ATP + 2-oxoglutarate + HCO3-
-
 Hydrogenobacter thermophilus TK-6 / IAM 12695 ADP + phosphate + oxalosuccinate
-
 ?
ATP + 2-oxoglutarate + HCO3- first step of the reductive carboxylation from 2-oxoglutarate to isocitrate. Oxalosuccinate, the product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 Hydrogenobacter thermophilus TK-6 / IAM 12695 ADP + phosphate + oxalosuccinate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70 80
-
 Hydrogenobacter thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 inactivation above Hydrogenobacter thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30.6
-
 2-oxoglutarate pH 8.5, 20°C Hydrogenobacter thermophilus
31.7
-
 ATP pH 8.5, 20°C Hydrogenobacter thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Hydrogenobacter thermophilus

Cofactor

Cofactor Comment Organism Structure
biotin enzyme contains biotin Hydrogenobacter thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Hydrogenobacter thermophilus isoelectric focusing
-
 4.9