Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.4.1.6 extracted from

  • Sluis, M.K.; Larsen, R.A.; Krum, J.G.; Anderson, R.; Metcalf, W.W.; Ensign, S.A.
    Biochemical, molecular, and genetic analyses of the acetone carboxylases from Xanthobacter autotrophicus strain Py2 and Rhodobacter capsulatus strain B10 (2002), J. Bacteriol., 184, 2969-2977.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.4.1.6

General Stability

General Stability Organism
4°C, enzyme in cell extract, stable for several days Xanthobacter autotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000042
-
 CO2
-
 Xanthobacter autotrophicus
0.0078
-
 acetone
-
 Xanthobacter autotrophicus
0.122
-
 ATP
-
 Xanthobacter autotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe contains 0.7 mol Fe per mol of enzyme Xanthobacter autotrophicus
K+ monovalent ion, K+ or NH4+, at concentration of 20-80 mM, required for optimal acetone carboxylase activity Xanthobacter autotrophicus
Mn contains 1.3 mol Mn per mol of enzyme Xanthobacter autotrophicus
NH4+ monovalent ion, K+ or NH4+, at concentration of 20-80 mM, required for optimal acetone carboxylase activity Xanthobacter autotrophicus
Zn contains 1.0 mol Zn per mol of enzyme Xanthobacter autotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19600
-
 alpha2beta2gamma2, 2 * 85300 + 2 * 78300 + 2 * 19600, MALDI-TOF Xanthobacter autotrophicus
23000
-
 alpha2beta2gamma2, 2 * 79000 + 2 * 68000 + 2 * 23000, SDS-PAGE Xanthobacter autotrophicus
68000
-
 alpha2beta2gamma2, 2 * 79000 + 2 * 68000 + 2 * 23000, SDS-PAGE Xanthobacter autotrophicus
78300
-
 alpha2beta2gamma2, 2 * 85300 + 2 * 78300 + 2 * 19600, MALDI-TOF Xanthobacter autotrophicus
79000
-
 alpha2beta2gamma2, 2 * 79000 + 2 * 68000 + 2 * 23000, SDS-PAGE Xanthobacter autotrophicus
85300
-
 alpha2beta2gamma2, 2 * 85300 + 2 * 78300 + 2 * 19600, MALDI-TOF Xanthobacter autotrophicus
353000
-
 gel filtration Xanthobacter autotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Xanthobacter autotrophicus not present at detectable levels in cells grown with carbon sources other than acetone ?
-
 ?

Organism

Organism UniProt Comment Textmining
Xanthobacter autotrophicus
-
-
-

Oxidation Stability

Oxidation Stability Organism
sensitive to oxygen Xanthobacter autotrophicus

Purification (Commentary)

Purification (Comment) Organism
-
 Xanthobacter autotrophicus

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetylacetone-grown cell
-
 Xanthobacter autotrophicus
-
additional information not present at detectable levels in cells grown with carbon sources other than acetone Xanthobacter autotrophicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.24
-
-
 Xanthobacter autotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetone + CO2 + ATP + H2O no detectable activity observed with CTP, ITP, GTP, XTP or pyrophosphate Xanthobacter autotrophicus acetoacetate + AMP + phosphate
-
 ?
additional information not present at detectable levels in cells grown with carbon sources other than acetone Xanthobacter autotrophicus ?
-
 ?

Subunits

Subunits Comment Organism
hexamer alpha2beta2gamma2, 2 * 79000 + 2 * 68000 + 2 * 23000, SDS-PAGE Xanthobacter autotrophicus
hexamer alpha2beta2gamma2, 2 * 85300 + 2 * 78300 + 2 * 19600, MALDI-TOF Xanthobacter autotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.33
-
 acetone
-
 Xanthobacter autotrophicus