BRENDA - Enzyme Database
show all sequences of 6.4.1.5

Geranoyl-CoA carboxylase: a novel biotin-containing enzyme in plants

Guan, X.; Diez, T.; Prasad, T.K.; Nikolau, B.J.; Wurtele, E.S.; Arch. Biochem. Biophys. 362, 12-21 (1999) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Avidin
complete inhibition, prevented by incubation of avidin with biotin prior to its addition
Zea mays
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0084
-
ATP
-
Zea mays
0.064
-
geranoyl-CoA
-
Zea mays
0.58
-
HCO3-
-
Zea mays
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
-
Zea mays
9536
-
plastid
84% of GCCase activity is recovered in plastids
Daucus carota
9536
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolute requirement
Zea mays
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
122000
-
x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis
Zea mays
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + geranoyl-CoA + HCO3-
Zea mays
plays an important role in isoprenoid catabolism
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
Zea mays
?
ATP + geranoyl-CoA + HCO3-
Glycine max
plays an important role in isoprenoid catabolism
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
Glycine max
?
ATP + geranoyl-CoA + HCO3-
Daucus carota
plays an important role in isoprenoid catabolism
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
Daucus carota
?
Organism
Organism
UniProt
Commentary
Textmining
Daucus carota
-
-
-
Glycine max
-
-
-
Zea mays
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
184fold, from leaves
Zea mays
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
specific activities in different developing embryos, effect of cotyledon development and cell growth on GCCase activity
Daucus carota
additional information
-
specific activities in several tissues of 4 and 15 days old seedlings
Glycine max
additional information
-
specific activities in several tissues of 7 days old seedlings
Zea mays
0.138
-
-
Zea mays
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + geranoyl-CoA + HCO3-
ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA
649418
Zea mays
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
formation of gamma-carboxygeranoyl-CoA
649418
Zea mays
?
ATP + geranoyl-CoA + HCO3-
ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA
649418
Glycine max
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
formation of gamma-carboxygeranoyl-CoA
649418
Glycine max
?
ATP + geranoyl-CoA + HCO3-
ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA
649418
Daucus carota
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
formation of gamma-carboxygeranoyl-CoA
649418
Daucus carota
?
ATP + geranoyl-CoA + HCO3-
plays an important role in isoprenoid catabolism
649418
Zea mays
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
649418
Zea mays
?
ATP + geranoyl-CoA + HCO3-
plays an important role in isoprenoid catabolism
649418
Glycine max
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
649418
Glycine max
?
ATP + geranoyl-CoA + HCO3-
plays an important role in isoprenoid catabolism
649418
Daucus carota
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
649418
Daucus carota
?
additional information
not: methylcrotonyl-CoA
649418
Zea mays
?
-
649418
Zea mays
?
Subunits
Subunits
Commentary
Organism
?
x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis
Zea mays
Synonyms
Synonyms
Commentary
Organism
GCCase
-
Zea mays
GCCase
-
Glycine max
GCCase
-
Daucus carota
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Zea mays
pH Range
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
active over a broad pH-range
Zea mays
Cofactor
Cofactor
Commentary
Organism
Structure
biotin
biotin-dependent enzyme
Zea mays
biotin
biotin-dependent enzyme
Glycine max
biotin
biotin-dependent enzyme
Daucus carota
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
biotin
biotin-dependent enzyme
Zea mays
biotin
biotin-dependent enzyme
Glycine max
biotin
biotin-dependent enzyme
Daucus carota
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Avidin
complete inhibition, prevented by incubation of avidin with biotin prior to its addition
Zea mays
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0084
-
ATP
-
Zea mays
0.064
-
geranoyl-CoA
-
Zea mays
0.58
-
HCO3-
-
Zea mays
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
-
Zea mays
9536
-
plastid
84% of GCCase activity is recovered in plastids
Daucus carota
9536
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolute requirement
Zea mays
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
122000
-
x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis
Zea mays
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + geranoyl-CoA + HCO3-
Zea mays
plays an important role in isoprenoid catabolism
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
Zea mays
?
ATP + geranoyl-CoA + HCO3-
Glycine max
plays an important role in isoprenoid catabolism
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
Glycine max
?
ATP + geranoyl-CoA + HCO3-
Daucus carota
plays an important role in isoprenoid catabolism
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
Daucus carota
?
Purification (Commentary) (protein specific)
Commentary
Organism
184fold, from leaves
Zea mays
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
specific activities in different developing embryos, effect of cotyledon development and cell growth on GCCase activity
Daucus carota
additional information
-
specific activities in several tissues of 4 and 15 days old seedlings
Glycine max
additional information
-
specific activities in several tissues of 7 days old seedlings
Zea mays
0.138
-
-
Zea mays
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + geranoyl-CoA + HCO3-
ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA
649418
Zea mays
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
formation of gamma-carboxygeranoyl-CoA
649418
Zea mays
?
ATP + geranoyl-CoA + HCO3-
ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA
649418
Glycine max
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
formation of gamma-carboxygeranoyl-CoA
649418
Glycine max
?
ATP + geranoyl-CoA + HCO3-
ATP-dependent carboxylation of the methyl group that is proximal to the carboxyl end of geranoyl-CoA
649418
Daucus carota
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
formation of gamma-carboxygeranoyl-CoA
649418
Daucus carota
?
ATP + geranoyl-CoA + HCO3-
plays an important role in isoprenoid catabolism
649418
Zea mays
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
649418
Zea mays
?
ATP + geranoyl-CoA + HCO3-
plays an important role in isoprenoid catabolism
649418
Glycine max
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
649418
Glycine max
?
ATP + geranoyl-CoA + HCO3-
plays an important role in isoprenoid catabolism
649418
Daucus carota
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
-
649418
Daucus carota
?
additional information
not: methylcrotonyl-CoA
649418
Zea mays
?
-
649418
Zea mays
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis
Zea mays
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Zea mays
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
active over a broad pH-range
Zea mays
Other publictions for EC 6.4.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
745854
Jurado
Structure and substrate selec ...
Pseudomonas fluorescens, Pseudomonas aeruginosa, Pseudomonas aeruginosa DSM 22644
Nat. Commun.
6
8986
2015
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692864
Aguilar
Substrate specificity of the 3 ...
Pseudomonas aeruginosa
J. Bacteriol.
190
4888-4893
2008
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1
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3
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2
1
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5
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1
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1
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3
1
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1
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1
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1
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1
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1
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3
1
1
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1
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-
671453
Aguilar
The atu and liu clusters are i ...
Pseudomonas aeruginosa
Appl. Environ. Microbiol.
72
2070-2079
2006
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-
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1
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9
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1
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1
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1
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671465
Foerster-Fromme
Identification of genes and pr ...
Pseudomonas aeruginosa
Appl. Environ. Microbiol.
72
4819-4828
2006
-
-
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1
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13
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1
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2
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1
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1
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-
662790
Hoschle
Methylcrotonyl-CoA and geranyl ...
Pseudomonas aeruginosa
Microbiology
151
3649-3656
2005
-
-
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2
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6
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2
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2
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2
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660754
Diaz-Perez
The gnyRDBHAL cluster is invol ...
Pseudomonas aeruginosa
Appl. Environ. Microbiol.
70
5102-5110
2004
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1
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4
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2
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2
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649484
Nikolau
Plant biotin-containing carbox ...
Pseudomonas sp., Zea mays
Arch. Biochem. Biophys.
414
211-222
2003
-
-
-
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3
1
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3
2
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4
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4
2
2
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2
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2
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3
1
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3
2
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4
2
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649418
Guan
Geranoyl-CoA carboxylase: a no ...
Daucus carota, Glycine max, Zea mays
Arch. Biochem. Biophys.
362
12-21
1999
-
-
-
-
-
-
1
3
2
1
1
3
-
15
-
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1
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-
4
-
7
1
3
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-
1
1
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3
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3
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1
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3
2
1
1
3
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1
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4
-
7
1
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1
1
-
-
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-
1886
Fall
3-Methylcrotonyl-CoA and geran ...
Pseudomonas citronellolis
Methods Enzymol.
71
791-799
1981
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3
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5
1
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3
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1
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1
1
5
1
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1
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1905
Cantwell
Biodegradation of acyclic isop ...
Pseudomonas aeruginosa, Pseudomonas citronellolis, Pseudomonas mendocina
J. Bacteriol.
135
324-333
1978
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4
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1888
Fall
Acyl-coenzyme A carboxylases. ...
Pseudomonas citronellolis
Biochemistry
16
4000-4005
1977
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1
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3
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1
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2
1
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1
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1
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1
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3
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1
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1
1
2
1
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1871
Hector
Multiple acyl-coenzyme A carbo ...
Pseudomonas citronellolis
Biochemistry
15
3465-3472
1976
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2
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1
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1
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1889
Hector
Evidence for distinct 3-methyl ...
Pseudomonas citronellolis
Biochem. Biophys. Res. Commun.
71
746-753
1976
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1904
Seubert
-
Untersuchungen über den bakte ...
Pseudomonas citronellolis
Biochem. Z.
338
265-275
1963
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1
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1
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1
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1
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4
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1
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1
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1
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1
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-