Literature summary for 6.4.1.4 extracted from

Diaz-Perez, C.; Rodriguez-Zavala, J.S.; Diaz-Perez, A.L.; Campos-Garcia, J.
Co-expression of alpha and beta subunits of the 3-methylcrotonyl-coenzyme A carboxylase from Pseudomonas aeruginosa (2012), World J. Microbiol. Biotechnol., 28, 1185-1191.

Search for more literature for 6.4.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
MCCase subunits are co-expressed in Escherichia coli in the same plasmid and purified in one step by affinity chromatography using the LiuD-His tag protein interacting with the LiuB-S tag recombinant protein	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structures diffracted to 1.5 A resolution of the Pseudomonas aeruginosa MCC (PaMCC) holoenzyme are shown, alone and in complex with coenzyme A. The structures show that the architecture and overall shape of PaMCC are strikingly different when compared to propionyl-CoA carboxylase	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.168	-	3-methylcrotonoyl-CoA	Vmax: 430 nmol/mg/min, pH and temperature not specified in the publication	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
490000	-	gel filtration	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9I299 and Q9I297	Q9I299, alpha-subunit and Q9I297, beta-subunit	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 3-methylcrotonoyl-CoA + HCO3-	-	Pseudomonas aeruginosa	ADP + phosphate + 3-methylglutaconyl-CoA	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	co-expressed subunits form an (alpha-beta)4 complex that suffer a modification of its oligomerization	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
3-methylcrotonyl coenzyme-A carboxylase	-	Pseudomonas aeruginosa
liuB	encoding beta subunit of MCCase	Pseudomonas aeruginosa
liuD	encoding alpha subunit of MCCase	Pseudomonas aeruginosa
MCCase	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	recombinant enzyme	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	recombinant enzyme	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)