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Literature summary for 6.4.1.4 extracted from
- Structural evidence for the involvement of the residues Ser187 and Tyr422 in substrate recognition in the 3-methylcrotonyl-coenzyme A carboxylase from Pseudomonas aeruginosa (2013), J. Biochem., 154, 291-297.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F417Y | beta-subunit mutant retains activity, kcat/Km increased compared to wild-type, kcat (3-methylcrotonoyl-CoA) increased compared to wild-type, Km (3-methylcrotonoyl-CoA) increased compared to wild-type | Pseudomonas aeruginosa |
| F417Y/G423A | beta-subunit mutant retains activity, kcat/Km similar to wild-type, kcat (3-methylcrotonoyl-CoA) increased compared to wild-type, Km (3-methylcrotonoyl-CoA) increased compared to wild-type | Pseudomonas aeruginosa |
| G423A | beta-subunit mutant retains activity, kcat/Km decreased compared to wild-type, kcat (3-methylcrotonoyl-CoA) decreased compared to wild-type, Km (3-methylcrotonoyl-CoA) slightly decreased compared to wild-type | Pseudomonas aeruginosa |
| R51A | beta-subunit mutant retains activity, kcat/Km decreased compared to wild-type, kcat (3-methylcrotonoyl-CoA) decreased compared to wild-type, Km (3-methylcrotonoyl-CoA) slightly increased compared to wild-type | Pseudomonas aeruginosa |
| S187A | beta-subunit mutant shows no enzymatic activity | Pseudomonas aeruginosa |
| Y422D | beta-subunit mutant shows no enzymatic activity | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.179 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant G423A, pH and temperature not specified in the publication | Pseudomonas aeruginosa |  |
| 0.216 | - |
3-methylcrotonoyl-CoA | wild-type, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 0.309 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant R51A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 0.315 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant F417Y/G423A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 0.514 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant F417Y, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q9I299 and Q9I297 | Q9I299, alpha-subunit and Q9I297, beta-subunit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Pseudomonas aeruginosa | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-methylcrotonyl-coenzyme A | - |
Pseudomonas aeruginosa |
| Pa-MCCase | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
3-methylcrotonoyl-CoA | beta-subunit beta-subunit mutant R51A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 0.66 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant G423A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 0.96 | - |
3-methylcrotonoyl-CoA | wild-type, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 1.45 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant F417Y/G423A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 2.76 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant F417Y, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.39 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant R51A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 3.68 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant G423A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 4.4 | - |
3-methylcrotonoyl-CoA | wild-type, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 4.6 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant F417Y/G423A, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 5.36 | - |
3-methylcrotonoyl-CoA | beta-subunit mutant F417Y, pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
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