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Literature summary for 6.4.1.4 extracted from

  • Chu, C.H.; Cheng, D.
    Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC) (2007), Protein Expr. Purif., 53, 421-427.
    View publication on PubMed
Search for more literature for 6.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
use of baculovirus system to express functionally active human recombinant 3-methylcrotonyl-CoA carboxylase Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 ATP 37°C, pH 8.0, recombinant enzyme Homo sapiens
0.074
-
 3-methylcrotonyl-CoA 37°C, pH 8.0, recombinant enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000 + 6 * 80000, 3-methylcrotonyl-CoA carboxylase is a heteromultimeric complex (MW: 500000 Da-800000 Da) that is composed of alpha and beta subunits (ratio 1:1) which are encoded by distinct genes. MCCCalpha is the larger subunit that has 725 amino acids, which contains the Met-Lys-Met biotin attachment domain near its COOH end. Human MCCCbeta is the small subunit that is composed of 563 amino acids, which functions as a carboxyltransferase, SDS-PAGE Homo sapiens
80000
-
 x * 60000 + 6 * 80000, 3-methylcrotonyl-CoA carboxylase is a heteromultimeric complex (MW: 500000 Da-800000 Da) that is composed of alpha and beta subunits (ratio 1:1) which are encoded by distinct genes. MCCCalpha is the larger subunit that has 725 amino acids, which contains the Met-Lys-Met biotin attachment domain near its COOH end. Human MCCCbeta is the small subunit that is composed of 563 amino acids, which functions as a carboxyltransferase, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-methylcrotonoyl-CoA + HCO3-
-
 Homo sapiens ADP + phosphate + 3-methylglutaconyl-CoA
-
 ?

Subunits

Subunits Comment Organism
multimer x * 60000 + 6 * 80000, 3-methylcrotonyl-CoA carboxylase is a heteromultimeric complex (MW: 500000 Da-800000 Da) that is composed of alpha and beta subunits (ratio 1:1) which are encoded by distinct genes. MCCCalpha is the larger subunit that has 725 amino acids, which contains the Met-Lys-Met biotin attachment domain near its COOH end. Human MCCCbeta is the small subunit that is composed of 563 amino acids, which functions as a carboxyltransferase, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
3-methylcrotonyl-CoA carboxylase
-
 Homo sapiens
MCCC
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
 ATP 37°C, pH 8.0, recombinant enzyme Homo sapiens
4
-
 3-methylcrotonyl-CoA 37°C, pH 8.0, recombinant enzyme Homo sapiens