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Literature summary for 6.4.1.4 extracted from
- 3-Methylcrotonyl-CoA carboxylase from Achromobacter (1981), Methods Enzymol., 71, 781-791.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 3-methylcrotonoyl-CoA protects against inactivation | Achromobacter sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
3-methylcrotonoyl-CoA | - |
Achromobacter sp. | |
| 0.083 | - |
ATP | - |
Achromobacter sp. | |
| 2.17 | - |
HCO3- | - |
Achromobacter sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ or Mn2+ required | Achromobacter sp. | |
| Mn2+ | Mn2+ or Mg2+ required | Achromobacter sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
4 * 78000, biotin-free subunit A, + 4 * 96000, biotin-containing subunit B | Achromobacter sp. |
| 96000 | - |
4 * 78000, biotin-free subunit A, + 4 * 96000, biotin-containing subunit B | Achromobacter sp. |
| 700000 | 760000 | - |
Achromobacter sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Achromobacter sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.36 | - |
- |
Achromobacter sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-methylcrotonoyl-CoA + HCO3- | r | Achromobacter sp. | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
| ATP + 3-methylcrotonoyl-CoA + HCO3- | the forward reaction is about 10times faster than the reverse reaction | Achromobacter sp. | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
| additional information | catalyzes ATP/ADP exchange reaction | Achromobacter sp. | ? | - |
? | |
| additional information | catalyzes ATP/diphosphate exchange reaction | Achromobacter sp. | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 4 * 78000, biotin-free subunit A, + 4 * 96000, biotin-containing subunit B | Achromobacter sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | 8.3 | - |
Achromobacter sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | enzyme contains biotin | Achromobacter sp. | |
| biotin | contains 4 molecules of biotin per enzyme complex | Achromobacter sp. | |
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Release 2023.1 (January 2023)
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