Literature summary for 6.4.1.2 extracted from

Rajamohan, F.; Marr, E.; Reyes, A.R.; Landro, J.A.; Anderson, M.D.; Corbett, J.W.; Dirico, K.J.; Harwood, J.H.; Tu, M.; Vajdos, F.F.
Structure-guided inhibitor design for human acetyl-coenzyme A carboxylase by interspecies active site conversion (2011), J. Biol. Chem., 286, 41510-41519.

Search for more literature for 6.4.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
humanized mutant of yeast CT domain is expressed in Escherichia coli	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of humanized mutant of yeast CT (yCT-H9) in complex with a human ACC2-selective small molecule inhibitor is determined	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
P1760S/I1762L/M1765V/E1919Q/P1920A/H1925F/Q2028E/M2030T/G2032E	humanized mutant of yeast CT domain is generated by replacing nine active site residues of yeast CT domain with corresponding human ACC2 CT domain residues. This humanized yeast CT domain (yCT-H9) exhibits an inhibitor sensitivity profile similar to that of human ACC while maintaining high recombinant expression yields and robust crystallizability	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q00955	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00000132	-	pH 8, 20°C	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + acetyl-CoA + HCO3-	-	Saccharomyces cerevisiae	ADP + phosphate + malonyl-CoA	-	?

Synonyms

Synonyms	Comment	Organism
ACC	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)