Literature summary for 6.4.1.2 extracted from

Alves, J.; Westling, L.; Peters, E.C.; Harris, J.L.; Trauger, J.W.
Cloning, expression, and enzymatic activity of Acinetobacter baumannii and Klebsiella pneumoniae acetyl-coenzyme A carboxylases (2011), Anal. Biochem., 417, 103-111.

Search for more literature for 6.4.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as a His-tagged fusion protein	Klebsiella pneumoniae
expressed in Escherichia coli as a His-tagged fusion protein	Acinetobacter baumannii

Inhibitors

Inhibitors	Comment	Organism	Structure
pseudopeptide pyrrolidinedione inhibitor	-	Acinetobacter baumannii
pseudopeptide pyrrolidinedione inhibitor	-	Klebsiella pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0088	-	acetyl-CoA	pH 8, 20°C	Acinetobacter baumannii
0.0487	-	acetyl-CoA	pH 8, 20°C	Klebsiella pneumoniae
0.083	-	ATP	pH 8, 20°C	Klebsiella pneumoniae
0.1037	-	ATP	pH 8, 20°C	Acinetobacter baumannii

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter baumannii	-	-	-
Klebsiella pneumoniae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Klebsiella pneumoniae
using Ni-NTA chromatography	Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + acetyl-CoA + HCO3-	-	Klebsiella pneumoniae	ADP + phosphate + malonyl-CoA	-	?
ATP + acetyl-CoA + HCO3-	-	Acinetobacter baumannii	ADP + phosphate + malonyl-CoA	-	?

Synonyms

Synonyms	Comment	Organism
ACCase	-	Klebsiella pneumoniae
ACCase	-	Acinetobacter baumannii
Acetyl-coenzyme A carboxylase	-	Klebsiella pneumoniae
Acetyl-coenzyme A carboxylase	-	Acinetobacter baumannii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
11.6	-	acetyl-CoA	co-substrate: ATP, HCO3-, pH 8, 20°C	Acinetobacter baumannii
30.1	-	acetyl-CoA	co-substrate: ATP, HCO3-, pH 8, 20°C	Klebsiella pneumoniae

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.000008	-	pH 8, 20°C	Klebsiella pneumoniae	pseudopeptide pyrrolidinedione inhibitor
0.000033	-	pH 8, 20°C	Acinetobacter baumannii	pseudopeptide pyrrolidinedione inhibitor

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Release 2023.1 (January 2023)