Literature summary for 6.4.1.2 extracted from

Kaushik, V.K.; Kavana, M.; Volz, J.M.; Weldon, S.C.; Hanrahan, S.; Xu, J.; Caplan, S.L.; Hubbard, B.K.
Characterization of recombinant human acetyl-CoA carboxylase-2 steady-state kinetics (2009), Biochim. Biophys. Acta, 1794, 961-967.

Search for more literature for 6.4.1.2

Activating Compound

Activating Compound	Comment	Organism	Structure
citrate	activates both by increasing kcat and kcat/KM for ATP and acetyl-CoA, has a putative regulatory function on ACC2	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
ATP	competitive inhibition versus ADP	Homo sapiens
HCO3-	competitive inhibition versus phosphate	Homo sapiens
malonyl-CoA	competitive inhibition versus acetyl-CoA	Homo sapiens
additional information	product inhibition analysis, inhibition patterns, overview	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetic analysis of ACC2, kinetic mechanism, overview	Homo sapiens
0.048	-	acetyl-CoA	pH 7.5, recombinant enzyme	Homo sapiens
0.125	-	ATP	pH 7.5, recombinant enzyme	Homo sapiens
2.1	-	HCO3-	pH 7.5, recombinant enzyme	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + acetyl-CoA + HCO3-	Homo sapiens	-	ADP + phosphate + malonyl-CoA	malonyl-CoA is a key metabolite in the fatty acid synthetic and oxidation pathways	ir
ATP + acetyl-CoA + HCO3- + H+	Homo sapiens	-	ADP + phosphate + malonyl-CoA	malonyl-CoA is a key metabolite in the fatty acid synthetic and oxidation pathways	ir

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Sf9 insect cells by ammonium sulfate fractionation and anion exchange chromatography	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + acetyl-CoA + hydrogencarbonate = ADP + phosphate + malonyl-CoA	the enzyme proceeds through a hybrid (two-site) random Ter Ter mechanism, one that likely involves a two-step reaction at the biotin carboxylase domain	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + acetyl-CoA + HCO3-	-	Homo sapiens	ADP + phosphate + malonyl-CoA	-	ir
ATP + acetyl-CoA + HCO3-	-	Homo sapiens	ADP + phosphate + malonyl-CoA	malonyl-CoA is a key metabolite in the fatty acid synthetic and oxidation pathways	ir
ATP + acetyl-CoA + HCO3- + H+	-	Homo sapiens	ADP + phosphate + malonyl-CoA	-	ir
ATP + acetyl-CoA + HCO3- + H+	-	Homo sapiens	ADP + phosphate + malonyl-CoA	malonyl-CoA is a key metabolite in the fatty acid synthetic and oxidation pathways	ir

Synonyms

Synonyms	Comment	Organism
ACC2	-	Homo sapiens
acetyl-CoA carboxylase-2	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.9	-	acetyl-CoA	pH 7.5, recombinant enzyme	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens
biotin	ACC2 is a biotinylated enzyme	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	product inhibition kinetics, detailed overview	Homo sapiens

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Release 2023.1 (January 2023)