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Literature summary for 6.4.1.2 extracted from

  • Hoja, U.; Marthol, S.; Hofmann, J.; Stegner, S.; Schulz, R.; Meier, S.; Greiner, E.; Schweizer, E.
    HFA1 encoding an organelle-specific acetyl-CoA carboxylase controls mitochondrial fatty acid synthesis in Saccharomyces cerevisiae (2004), J. Biol. Chem., 279, 21779-21786.
    View publication on PubMed
Search for more literature for 6.4.1.2

Cloned(Commentary)

Cloned (Comment) Organism
gene hfa1, expression analysis, complementation of an acc1-defective mutant yeast strain Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information mutants of genes HFA1 and ACC1 shows different phenotypes and the enzymes cannot complement each other, the mitochondrial enzyme Hfa1p disruption mutant strain shows about 90% reduced lipoic acid concentration and does not grow on lactate or glycerol, complementation of hfa1 null mutants require full length HFA1 DNA including the mitochondrial targeting sequence, without the sequence the cDNA encoding the mitochondrial enzyme can complement the cytoplasmic acc1-defective mutant, overview Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm Acc1p Saccharomyces cerevisiae 5737
-
mitochondrion Hfa1p is a mitochondrion-specific enzyme and contains a canonical mitochondrial targeting sequence with a matrix protease cleavage site Saccharomyces cerevisiae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetyl-CoA + HCO3- Saccharomyces cerevisiae enzymes Hfa1p and Acc1p are involved in mitochondrial and cytoplasmic fatty acid synthesis, respectively, Hfa1p is responsible for production of malonyl-CoA for intraorganellar fatty acid and lipoic acid synthesis ADP + malonyl-CoA + phosphate
-
 ?
ATP + acetyl-CoA + HCO3- Saccharomyces cerevisiae X2180-1A enzymes Hfa1p and Acc1p are involved in mitochondrial and cytoplasmic fatty acid synthesis, respectively, Hfa1p is responsible for production of malonyl-CoA for intraorganellar fatty acid and lipoic acid synthesis ADP + malonyl-CoA + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 genes HFA1 and ACC1
-
Saccharomyces cerevisiae X2180-1A
-
 genes HFA1 and ACC1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA + HCO3-
-
 Saccharomyces cerevisiae ADP + malonyl-CoA + phosphate
-
 ?
ATP + acetyl-CoA + HCO3- enzymes Hfa1p and Acc1p are involved in mitochondrial and cytoplasmic fatty acid synthesis, respectively, Hfa1p is responsible for production of malonyl-CoA for intraorganellar fatty acid and lipoic acid synthesis Saccharomyces cerevisiae ADP + malonyl-CoA + phosphate
-
 ?
ATP + acetyl-CoA + HCO3-
-
 Saccharomyces cerevisiae X2180-1A ADP + malonyl-CoA + phosphate
-
 ?
ATP + acetyl-CoA + HCO3- enzymes Hfa1p and Acc1p are involved in mitochondrial and cytoplasmic fatty acid synthesis, respectively, Hfa1p is responsible for production of malonyl-CoA for intraorganellar fatty acid and lipoic acid synthesis Saccharomyces cerevisiae X2180-1A ADP + malonyl-CoA + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Acc1p
-
 Saccharomyces cerevisiae
Hfa1p
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae