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Literature summary for 6.4.1.2 extracted from
- Purification and characterization of maize leaf acetyl-coenzyme A carboxylase (1984), Arch. Biochem. Biophys., 228, 86-96.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Zea mays |  |
| AMP | slight | Zea mays | |
| Li+ | - |
Zea mays | |
| malonyl-CoA | - |
Zea mays | |
| additional information | addition of cations in presence of Mg2+ inhibits | Zea mays | |
| oxaloacetate | weak | Zea mays | |
| phosphoenolpyruvate | weak | Zea mays | |
| propionyl-CoA | below 0.1 mM almost as effective as substrate as acetyl-CoA, inhibitory at higher concentrations | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
acetyl-CoA | - |
Zea mays | |
| 1.4 | - |
HCO3- | - |
Zea mays | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cs+ | greater activation at 1 mM HCO3- than at 10 mM HCO3- | Zea mays | |
| K+ | greatest activation at 12-15 mM K+, at HCO3- concentration of 0.18 mM, 1 mM, and 10 mM | Zea mays | |
| Mg2+ | maximal activity at 3 mM Mg2+ with 1 mM ATP and at 4 mM Mg2+ with 3 mM ATP | Zea mays | |
| Mg2+ | free Mg2+ activates | Zea mays | |
| Mn2+ | maximal activity at 1 mM Mn2+ with 1 mM ATP. Maximal activity is 25% of that observed with Mg2+ | Zea mays | |
| NH4+ | greater activation at 1 mM HCO3- than at 10 mM HCO3- | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 500000 | - |
gel filtration | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acetyl-CoA carboxylase I and acetyl-CoA carboxylase II | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.844 | - |
- |
Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetyl-CoA + HCO3- | - |
Zea mays | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + butyryl-CoA + HCO3- | no activity | Zea mays | ADP + ethylmalonyl-CoA + phosphate | - |
? | |
| ATP + propionyl-CoA + HCO3- | 45% of the activity compared to acetyl-CoA | Zea mays | ADP + phosphate + methylmalonyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Zea mays |
| octamer | 8 * 60000-610000, SDS-PAGE | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Zea mays |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 9.8 | 50% of maximal activity at pH 7.4 and 9.8 | Zea mays |
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