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Literature summary for 6.4.1.1 extracted from

  • Choosangtong, K.; Sirithanakorn, C.; Adina-Zada, A.; Wallace, J.C.; Jitrapakdee, S.; Attwood, P.V.
    Residues in the acetyl CoA binding site of pyruvate carboxylase involved in allosteric regulation (2015), FEBS Lett., 589, 2073-2079 .
    View publication on PubMed
Search for more literature for 6.4.1.1

Activating Compound

Activating Compound Comment Organism Structure
acetyl-CoA interacts directly with residues R469 and D471 Rhizobium etli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rhizobium etli

Protein Variants

Protein Variants Comment Organism
D1018A mutant has increased activity in the absence of acetyl-CoA, but not in its presence Rhizobium etli
D471A mutant exhibits no acetyl CoA-activation Rhizobium etli
E1027A mutant has increased activity in the absence of acetyl-CoA, but not in its presence Rhizobium etli
E1027R mutant has increased activity in the absence of acetyl-CoA, but not in its presence Rhizobium etli
R469K mutant shows increased enzymic activity in the presence and absence of acetyl-CoA Rhizobium etli
R469S mutant shows increased enzymic activity in the presence and absence of acetyl-CoA Rhizobium etli

Organism

Organism UniProt Comment Textmining
Rhizobium etli Q2K340
-
-
Rhizobium etli ATCC 51251 Q2K340
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + HCO3-
-
 Rhizobium etli ADP + phosphate + oxaloacetate
-
 ?
ATP + pyruvate + HCO3-
-
 Rhizobium etli ATCC 51251 ADP + phosphate + oxaloacetate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.105
-
 pyruvate mutant D471A, pH 7.8, 30°C, presence of acetyl-CoA Rhizobium etli
0.11
-
 pyruvate mutant D471A, pH 7.8, 30°C Rhizobium etli
0.18
-
 pyruvate wild-type, pH 7.8, 30°C, Hill coefficient 2.7 Rhizobium etli
0.68
-
 pyruvate mutant R469K, pH 7.8, 30°C, Hill coefficient 0.9 Rhizobium etli
1.18
-
 pyruvate mutant E1027R, pH 7.8, 30°C, Hill coefficient 2.3 Rhizobium etli
1.61
-
 pyruvate mutant R469S, pH 7.8, 30°C, Hill coefficient 2.3 Rhizobium etli
2.17
-
 pyruvate mutant D1018A, pH 7.8, 30°C, Hill coefficient 2.1 Rhizobium etli
2.71
-
 pyruvate mutant E1027R, pH 7.8, 30°C, Hill coefficient 2.3, presence of acetyl-CoA Rhizobium etli
3.94
-
 pyruvate mutant E1027A, pH 7.8, 30°C, Hill coefficient 3.0 Rhizobium etli
6.05
-
 pyruvate mutant R469K, pH 7.8, 30°C, Hill coefficient 0.9, presence of acetyl-CoA Rhizobium etli
11.3
-
 pyruvate mutant R469S, pH 7.8, 30°C, Hill coefficient 2.3, presence of acetyl-CoA Rhizobium etli
12.3
-
 pyruvate mutant D1018A, pH 7.8, 30°C, Hill coefficient 2.1, presence of acetyl-CoA Rhizobium etli
14.9
-
 pyruvate mutant E1027A, pH 7.8, 30°C, Hill coefficient 3.0, presence of acetyl-CoA Rhizobium etli
17.6
-
 pyruvate wild-type, pH 7.8, 30°C, Hill coefficient 2.7, presence of acetyl-CoA Rhizobium etli