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Literature summary for 6.4.1.1 extracted from

  • Marlier, J.F.; Cleland, W.W.; Zeczycki, T.N.
    Oxamate is an alternative substrate for pyruvate carboxylase from Rhizobium etli (2013), Biochemistry, 52, 2888-2894.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetyl-CoA
-
Rhizobium etli
oxamate oxamate acts as a carboxyl acceptor, forming a carbamylated oxamate product and thereby accelerating the enzymatic decarboxylation reaction Rhizobium etli

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Rhizobium etli

Organism

Organism UniProt Comment Textmining
Rhizobium etli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Rhizobium etli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + HCO3-
-
Rhizobium etli ADP + phosphate + oxaloacetate
-
?

Synonyms

Synonyms Comment Organism
pyruvate carboxylase
-
Rhizobium etli
RePC
-
Rhizobium etli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rhizobium etli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.056
-
oxaloacetate pH 7.5, 25°C, 0.25 mM oxamate Rhizobium etli
0.076
-
oxaloacetate pH 7.5, 25°C, 0.5 mM oxamate Rhizobium etli
0.151
-
oxaloacetate pH 7.5, 25°C, 0.75 mM oxamate Rhizobium etli
0.2
-
oxaloacetate pH 7.5, 25°C, 1 mM oxamate Rhizobium etli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Rhizobium etli