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Literature summary for 6.4.1.1 extracted from
- Protein engineering of pyruvate carboxylase (2004), Eur. J. Biochem., 271, 1391-1400.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| two polypeptide chain: PC-(BC) and PC-(CT+BCCP) | Geobacillus thermodenitrificans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | polypeptide chain is divided into two chains, between the biotin carboxylase and carboxyltransferase domains, resulting in two proteins PC-(BC) and PC-(CT+BCCP) with retained enzyme activity | Geobacillus thermodenitrificans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
pyruvate | pH 8, 30°C | Geobacillus thermodenitrificans |  |
| 0.46 | 0.87 | ATP | pH 8, 30°C | Geobacillus thermodenitrificans | |
| 0.54 | 1.03 | ATP | pH 8, 30°C, ATP-cleavage in absence of pyruvate | Geobacillus thermodenitrificans | |
| 29.9 | - |
HCO3- | pH 8, 30°C | Geobacillus thermodenitrificans | |
| 62.2 | - |
HCO3- | pH 8, 30°C, ATP-cleavage in absence of pyruvate | Geobacillus thermodenitrificans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51400 | - |
chain PC-(BC), SDS-PAGE, monomeric form | Geobacillus thermodenitrificans |
| 65000 | - |
chain PC-(CT+BCCP), SDS-PAGE, monomeric form | Geobacillus thermodenitrificans |
| 66200 | - |
chain PC-(CT+BCCP), gel filtration, monomeric form | Geobacillus thermodenitrificans |
| 81900 | - |
chain PC-(BC), gel filtration, dimeric form | Geobacillus thermodenitrificans |
| 128000 | - |
intact enzyme, SDS-PAGE, monomeric form | Geobacillus thermodenitrificans |
| 128200 | - |
chain PC-(CT+BCCP), gel filtration, dimeric form | Geobacillus thermodenitrificans |
| 501300 | - |
intact enzyme, gel filtration, tetrameric form | Geobacillus thermodenitrificans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + HCO3- | Geobacillus thermodenitrificans | - |
ADP + phosphate + oxaloacetate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermodenitrificans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus thermodenitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + HCO3- | - |
Geobacillus thermodenitrificans | ADP + phosphate + oxaloacetate | - |
r | |
| ATP + pyruvate + HCO3- | intact enzyme and PC-(BC) chain are able to cleave ATP in the presence of free D-biotin and absence of pyruvate | Geobacillus thermodenitrificans | ADP + phosphate + oxaloacetate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | PC-(BC) exists as a monomer and as a dimer, (CT+BCCP) exists mostly as a dimer, gel filtration | Geobacillus thermodenitrificans |
| monomer | PC-(BC) exists as a monomer and as a dimer, gel filtration | Geobacillus thermodenitrificans |
| tetramer | 4 * 128500-137000, SDS-PAGE, gel filtration, intact enzyme | Geobacillus thermodenitrificans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | - |
Geobacillus thermodenitrificans | |
| additional information | enzyme activities of PC-(BC) and PC-(CT+BCCP) are not dependent on acetyl-CoA | Geobacillus thermodenitrificans |
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Release 2023.1 (January 2023)
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