Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | inactive in absence of acetyl-CoA | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitively with ATP | Geobacillus stearothermophilus | |
ATP | - |
Geobacillus stearothermophilus | |
Avidin | - |
Geobacillus stearothermophilus | |
L-Asp | antagonizes activation by acetyl-CoA | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.128 | - |
pyruvate | - |
Geobacillus stearothermophilus | |
0.33 | - |
HCO3- | - |
Geobacillus stearothermophilus | |
0.38 | - |
ATP | - |
Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates | Geobacillus stearothermophilus | |
K+ | Km: 20 mM | Geobacillus stearothermophilus | |
Mg2+ | activates | Geobacillus stearothermophilus | |
Mg2+ | the presence of MgCl2 in excess of ATP produces a more than 3fold activation | Geobacillus stearothermophilus | |
Mn2+ | 3fold activation in presence of MnCl2 equimolar in concentration to ATP | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31.5 | - |
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
4°C, stable for at least 3 months | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | - |
Geobacillus stearothermophilus | ADP + phosphate + oxaloacetate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Tris hydrochloride, KCl or a mixture of MgCl2 and ATP protects against heat inactivation | Geobacillus stearothermophilus |
55 | - |
half-life: 9 min. A mixture of Tris-HCl, potassium bicarbonate, sodium pyruvate, ATP, MgC2l, and acetyl-CoA at concentrations similar to those used in the enzyme assay, protect completely for at least 30 min | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
in presence of MnCl2 | Geobacillus stearothermophilus |
8.3 | - |
in presence of MgCl2 | Geobacillus stearothermophilus |