Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.4.1.1 extracted from

  • Cazzulo, J.J.; Sundaram, T.K.; Kornberg, H.L.
    Properties and regulation of pyruvate carboxylase from Bacillus stearothermophilus (1970), Proc. R. Soc. Lond. B Biol. Sci., 176, 1-19.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetyl-CoA inactive in absence of acetyl-CoA Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
ADP competitively with ATP Geobacillus stearothermophilus
ATP
-
Geobacillus stearothermophilus
Avidin
-
Geobacillus stearothermophilus
L-Asp antagonizes activation by acetyl-CoA Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.128
-
pyruvate
-
Geobacillus stearothermophilus
0.33
-
HCO3-
-
Geobacillus stearothermophilus
0.38
-
ATP
-
Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Geobacillus stearothermophilus
K+ Km: 20 mM Geobacillus stearothermophilus
Mg2+ activates Geobacillus stearothermophilus
Mg2+ the presence of MgCl2 in excess of ATP produces a more than 3fold activation Geobacillus stearothermophilus
Mn2+ 3fold activation in presence of MnCl2 equimolar in concentration to ATP Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31.5
-
-
Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
4°C, stable for at least 3 months Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + HCO3-
-
Geobacillus stearothermophilus ADP + phosphate + oxaloacetate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Tris hydrochloride, KCl or a mixture of MgCl2 and ATP protects against heat inactivation Geobacillus stearothermophilus
55
-
half-life: 9 min. A mixture of Tris-HCl, potassium bicarbonate, sodium pyruvate, ATP, MgC2l, and acetyl-CoA at concentrations similar to those used in the enzyme assay, protect completely for at least 30 min Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
in presence of MnCl2 Geobacillus stearothermophilus
8.3
-
in presence of MgCl2 Geobacillus stearothermophilus