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Literature summary for 6.3.5.7 extracted from

  • Hadd, A.; Perona, J.J.
    Coevolution of specificity determinants in eukaryotic glutamyl- and glutaminyl-tRNA synthetases (2014), J. Mol. Biol., 426, 3619-3633 .
    View publication on PubMed
Search for more literature for 6.3.5.7

Protein Variants

Protein Variants Comment Organism
additional information construction of an an N-terminal deletion mutant lacking amino acids 1-186 corresponding to the eukaryote-specific protein domains. The domains substantially influence amino acid binding, tRNA binding and aminoacylation efficiency, but they play no role in either specific nucleotide readout or discrimination against noncognate tRNA Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 L-glutamine wild-type, pH 7.5, 37°C Saccharomyces cerevisiae
0.19
-
 L-glutamyl-tRNAGln wild-type, pH 7.5, 37°C Saccharomyces cerevisiae
1.2
-
 L-glutamine N-terminal deletion mutant, pH 7.5, 37°C Saccharomyces cerevisiae
5.6
-
 L-glutamyl-tRNAGln N-terminal deletion mutant, pH 7.5, 37°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P13188
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamyl-tRNAGln + L-glutamine
-
 Saccharomyces cerevisiae ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
 ?
ATP + L-glutamyl-tRNAGln + L-glutamine
-
 Saccharomyces cerevisiae ATCC 204508 / S288c ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
Gln4
-
 Saccharomyces cerevisiae
GlnRS
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
 L-glutamine wild-type, pH 7.5, 37°C Saccharomyces cerevisiae
1.7
-
 L-glutamine N-terminal deletion mutant, pH 7.5, 37°C Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism eukaryotic tRNAGln and tRNAGlu recognition determinants are found in equivalent positions of glutaminyl-tRNA synthetase and glutamyl-tRNA synthetase, respectively, and are mutually exclusive to a significant degree, with key nucleotides located adjacent to portions of the protein structure that differentiated during the evolution of archaeal nondiscriminating luRS to GlnRS Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4
-
 L-glutamine wild-type, pH 7.5, 37°C Saccharomyces cerevisiae
1.7
-
 L-glutamine N-terminal deletion mutant, pH 7.5, 37°C Saccharomyces cerevisiae