Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the presence of 0.0066 mM GluRS2 increases the KM of the enzyme for Glu-tRNAGln nearly 4fold | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00042 | - |
Glu-tRNAGln | in the absence of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C | Helicobacter pylori | |
0.00097 | - |
Glu-tRNAGln | in the presence of equimolar amounts of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C | Helicobacter pylori | |
0.00134 | - |
Glu-tRNAGln | in the presence of 440fold excess of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Glu-tRNAGln + L-glutamine + H2O | Helicobacter pylori | - |
ADP + phosphate + Gln-tRNAGln + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, Ni-NTA resin column chromatography, and Superdex 200 gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Glu-tRNAGln + L-glutamine + H2O | - |
Helicobacter pylori | ADP + phosphate + Gln-tRNAGln + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GatCAB | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
Glu-tRNAGln | in the absence of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C | Helicobacter pylori | |
0.21 | - |
Glu-tRNAGln | in the presence of 440fold excess of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C | Helicobacter pylori | |
0.23 | - |
Glu-tRNAGln | in the presence of equimolar amounts of GluRS2, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 12 mM MgCl2, at 37°C | Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Helicobacter pylori |