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Literature summary for 6.3.5.5 extracted from

  • Simmons, A.J.; Rawls, J.M.; Piskur, J.; Davidson, J.N.
    A mutation that uncouples allosteric regulation of carbamyl phosphate synthetase in Drosophila (1999), J. Mol. Biol., 287, 277-285.
    View publication on PubMed
Search for more literature for 6.3.5.5

Protein Variants

Protein Variants Comment Organism
additional information supressor of black mutation, Su(b), which causes a loss of UTP feedback inhibition, is a missense mutation resulting in a glutamate to lysine substitution within the second ATP binding site of enzyme Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
UTP slight UTP inhibition of SU(b) mutant enzyme is probably due to the chelating of Mg2+ by UTP Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 L-glutamine pH 7.4, 30°C, Su(b) mutant Drosophila melanogaster
0.057
-
 L-glutamine pH 7.4, 30°C, wild-type enzyme Drosophila melanogaster
2.4
-
 HCO3- pH 7.4, 30°C, Su(b) mutant Drosophila melanogaster
3
-
 HCO3- pH 7.4, 30°C, wild-type enzyme Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster P05990 supressor of black mutation
-

Purification (Commentary)

Purification (Comment) Organism
partial Drosophila melanogaster

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
 Drosophila melanogaster 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?