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Literature summary for 6.3.5.5 extracted from

  • Kim, J.; Howell, S.; Huang, X.; Raushel, F.M.
    Structural defects within the carbamate tunnel of carbamoyl phosphate synthetase (2002), Biochemistry, 41, 12575-12581.
    View publication on PubMed
Search for more literature for 6.3.5.5

Protein Variants

Protein Variants Comment Organism
additional information A series of mutants created and probes the direct transport of carbamate through the carbamate tunnel, the partial reactions of enzyme have not been significantly impaired by these mutations, and thus, the catalytic machinery at the individual active site has not been functionally pertured Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information comparison of KM of partial reactions of wild-type and mutant enzymes Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 alpha,beta, 1 * 42000 + 1 * 118000, the ammonia tunnel is responsible for the migration of ammonia, that is produced at the active site of the small subunit and migrates to the large subunit, the carbamate tunnel connects the two active sites contained within the carboxy phosphate and carbamoyl phosphate domains of the large subunit Escherichia coli
118000
-
 alpha,beta, 1 * 42000 + 1 * 118000, the ammonia tunnel is responsible for the migration of ammonia, that is produced at the active site of the small subunit and migrates to the large subunit, the carbamate tunnel connects the two active sites contained within the carboxy phosphate and carbamoyl phosphate domains of the large subunit Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ATP + L-Gln + HCO3- Escherichia coli
-
 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2 ATP + L-glutamine + hydrogencarbonate + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate catalytic mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
 Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?

Subunits

Subunits Comment Organism
heterodimer alpha,beta, 1 * 42000 + 1 * 118000, the ammonia tunnel is responsible for the migration of ammonia, that is produced at the active site of the small subunit and migrates to the large subunit, the carbamate tunnel connects the two active sites contained within the carboxy phosphate and carbamoyl phosphate domains of the large subunit Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information comparison of kcat of partial reactions of wild-type and mutant enzymes Escherichia coli