Literature summary for 6.3.5.5 extracted from

Guy, H.I.; Rotgeri, A.; Evans, D.R.
Activation by fusion of the glutaminase and synthetase subunits of Escherichia coli carbamyl-phosphate synthetase (1997), J. Biol. Chem., 272, 19913-19918.

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Protein Variants

Protein Variants	Comment	Organism
additional information	the 120000 MW synthetase subunit and the 40000 MW glutaminase subunit, in which the intergenic region between the contiguous carA and carB genes is deleted and the sequence encoding the carbamoyl-phosphate synthase subunits is fused in frame. The resulting fusion protein is activated 10fold relative to the native protein, it is responsive to the allosteric activator Orn, and cannot use NH4+ as a nitrogen donor. The functional linkage that coordinates the rate of glutamine hydrolysis with the activation of bicarbonate is abolished	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	1 * 40000, glutaminase subunit, + 1 * 120000, synthase subunit. The synthase subunit consists of two homologous domains, CPS.A and CPS.B, which catalyze the two different ATP-dependent partial reactions involved in carbamoyl-phosphate synthesis	Escherichia coli
120000	-	1 * 40000, glutaminase subunit, + 1 * 120000, synthase subunit. The synthase subunit consists of two homologous domains, CPS.A and CPS.B, which catalyze the two different ATP-dependent partial reactions involved in carbamoyl-phosphate synthesis	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 ATP + L-Gln + HCO3-	-	Escherichia coli	2 ADP + phosphate + L-Glu + carbamoyl phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	1 * 40000, glutaminase subunit, + 1 * 120000, synthase subunit. The synthase subunit consists of two homologous domains, CPS.A and CPS.B, which catalyze the two different ATP-dependent partial reactions involved in carbamoyl-phosphate synthesis	Escherichia coli

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Release 2023.1 (January 2023)