Literature summary for 6.3.5.4 extracted from

Bai, L.; Jiang, R.; Shan, J.; Guo, L.; Zhang, Y.; Zhang, R.; Li, Y.
Purification, characterization and functional analysis of asparagine synthetase encoding by ste10 gene in Ebosin biosynthesis of Streptomyces sp. 139 (2008), Enzyme Microb. Technol., 42, 548-553.

No PubMed abstract available

Search for more literature for 6.3.5.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptomyces sp.

Protein Variants

Protein Variants	Comment	Organism
additional information	after ste10 gene knock-out, the monosaccharide composition of the exopolysaccharide produced by the mutant is changed in comparison with that of native exopolysaccharide Ebosin while its antagonist activity for IL-1R decreases significantly	Streptomyces sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.9	-	L-Asp	pH 7.4, 37°C	Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	x * 70000, SDS-PAGE and calculated, His-tagged protein	Streptomyces sp.

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp.	Q8KN11	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Streptomyces sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-Asp + L-Gln	-	Streptomyces sp.	AMP + diphosphate + Asn + Glu	-	?

Subunits

Subunits	Comment	Organism
?	x * 70000, SDS-PAGE and calculated, His-tagged protein	Streptomyces sp.

Synonyms

Synonyms	Comment	Organism
Ste10	-	Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Streptomyces sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	-	Streptomyces sp.

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Release 2023.1 (January 2023)