Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycerol | required for optimum activity | Pisum sativum | |
glycerol | required for optimum activity | Glycine max | |
glycerol | required for optimum activity | Lupinus albus | |
additional information | 1 mM aminooxyacetic acid increases AS activity in crude extract by inhibiting various aminotransferases and preventing the assimilation of aspartate into the TCA cycle | Pisum sativum |
General Stability | Organism |
---|---|
unstable enzyme, 20-25% v/v glycerol and 65-70 mM 2-mercaptoethanol stabilize during storage at low temperatures | Pisum sativum |
unstable enzyme, 20-25% v/v glycerol and 65-70 mM 2-mercaptoethanol stabilize during storage at low temperatures | Glycine max |
unstable enzyme, 20-25% v/v glycerol and 65-70 mM 2-mercaptoethanol stabilize during storage at low temperatures | Lupinus albus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-asparagine | product inhibition | Lupinus albus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pisum sativum | primary enzyme responsible for asparagine synthesis | ? | - |
? | |
additional information | Glycine max | primary enzyme responsible for asparagine synthesis | ? | - |
? | |
additional information | Lupinus albus | primary enzyme responsible for asparagine synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
Merr. v. DP3588 | - |
Lupinus albus | - |
v. Victoria | - |
Pisum sativum | - |
v. Little Marvel, dark-grown | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | etiolated | Glycine max | - |
cotyledon | etiolated | Lupinus albus | - |
leaf | first leaves, etiolated | Pisum sativum | - |
Storage Stability | Organism |
---|---|
-25°C, semicrude extract, 9-10 days, 50% loss of activity | Lupinus albus |
-80°C, semicrude extract, over 3 weeks, 30% loss of activity | Lupinus albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine | ATP-dependent, the amine group of Gln is transferred directly to Asp | Pisum sativum | AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
ATP + L-aspartate + L-glutamine | ATP-dependent, the amine group of Gln is transferred directly to Asp | Glycine max | AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
ATP + L-aspartate + L-glutamine | ATP-dependent, the amine group of Gln is transferred directly to Asp, maximum activity with 1 mM Gln and 3-10 mM ATP in the assay | Lupinus albus | AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
additional information | primary enzyme responsible for asparagine synthesis | Pisum sativum | ? | - |
? | |
additional information | primary enzyme responsible for asparagine synthesis | Glycine max | ? | - |
? | |
additional information | primary enzyme responsible for asparagine synthesis | Lupinus albus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AS | - |
Pisum sativum |
AS | - |
Glycine max |
AS | - |
Lupinus albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pisum sativum |
25 | - |
assay at | Glycine max |
25 | - |
assay at | Lupinus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pisum sativum |
7.5 | - |
assay at | Glycine max |
7.5 | - |
assay at | Lupinus albus |