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Literature summary for 6.3.5.4 extracted from

  • Romagni, J.G.; Dayan, F.E.
    Measuring asparagine synthetase activity in crude plant extracts (2000), J. Agric. Food Chem., 48, 1692-1696.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
glycerol required for optimum activity Pisum sativum
glycerol required for optimum activity Glycine max
glycerol required for optimum activity Lupinus albus
additional information 1 mM aminooxyacetic acid increases AS activity in crude extract by inhibiting various aminotransferases and preventing the assimilation of aspartate into the TCA cycle Pisum sativum

General Stability

General Stability Organism
unstable enzyme, 20-25% v/v glycerol and 65-70 mM 2-mercaptoethanol stabilize during storage at low temperatures Pisum sativum
unstable enzyme, 20-25% v/v glycerol and 65-70 mM 2-mercaptoethanol stabilize during storage at low temperatures Glycine max
unstable enzyme, 20-25% v/v glycerol and 65-70 mM 2-mercaptoethanol stabilize during storage at low temperatures Lupinus albus

Inhibitors

Inhibitors Comment Organism Structure
L-asparagine product inhibition Lupinus albus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pisum sativum primary enzyme responsible for asparagine synthesis ?
-
?
additional information Glycine max primary enzyme responsible for asparagine synthesis ?
-
?
additional information Lupinus albus primary enzyme responsible for asparagine synthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
Merr. v. DP3588
-
Lupinus albus
-
v. Victoria
-
Pisum sativum
-
v. Little Marvel, dark-grown
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon etiolated Glycine max
-
cotyledon etiolated Lupinus albus
-
leaf first leaves, etiolated Pisum sativum
-

Storage Stability

Storage Stability Organism
-25°C, semicrude extract, 9-10 days, 50% loss of activity Lupinus albus
-80°C, semicrude extract, over 3 weeks, 30% loss of activity Lupinus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + L-glutamine ATP-dependent, the amine group of Gln is transferred directly to Asp Pisum sativum AMP + diphosphate + L-asparagine + L-glutamate
-
?
ATP + L-aspartate + L-glutamine ATP-dependent, the amine group of Gln is transferred directly to Asp Glycine max AMP + diphosphate + L-asparagine + L-glutamate
-
?
ATP + L-aspartate + L-glutamine ATP-dependent, the amine group of Gln is transferred directly to Asp, maximum activity with 1 mM Gln and 3-10 mM ATP in the assay Lupinus albus AMP + diphosphate + L-asparagine + L-glutamate
-
?
additional information primary enzyme responsible for asparagine synthesis Pisum sativum ?
-
?
additional information primary enzyme responsible for asparagine synthesis Glycine max ?
-
?
additional information primary enzyme responsible for asparagine synthesis Lupinus albus ?
-
?

Synonyms

Synonyms Comment Organism
AS
-
Pisum sativum
AS
-
Glycine max
AS
-
Lupinus albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pisum sativum
25
-
assay at Glycine max
25
-
assay at Lupinus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pisum sativum
7.5
-
assay at Glycine max
7.5
-
assay at Lupinus albus