Literature summary for 6.3.5.4 extracted from

Larsen, T.M.; Boehlein, S.K.; Schuster, S.M.; Richards, N.G.; Thoden, J.B.; Holden, H.M.; Rayment, I.
Three-dimensional structure of Escherichia coli asparagine synthetase B: a short journey from substrate to product (1999), Biochemistry, 38, 16146-16157.

Crystallization (Commentary)

Crystallization (Comment)	Organism
AS-B is dialyzed against 20 mM Bis-Tris, pH 6.5, 150 mM NaCl, 0.5 mM EDTA, and 2 mM dithiothreitol, crystals are grown by hanging drop vapor diffusion at 4°C, AS-B solution is adjusted to 6.5 mg/ml and contains 10 mM MgCl2, 5 mM glutamine, and 10 mM AMP, 0.01 ml of protein solution are mixed with 0.01 ml precipitant solution containing 14% polyethylene glycol 8000 and 50 mM HEPES, pH 7.0, these droplets are suspended against wells containing 15% polyethylene glycol 8000 and 50 mM HEPES, pH 7.0, crystals diffract to 2.0 A	Escherichia coli

Crystallization (Comment)

Organism

AS-B is dialyzed against 20 mM Bis-Tris, pH 6.5, 150 mM NaCl, 0.5 mM EDTA, and 2 mM dithiothreitol, crystals are grown by hanging drop vapor diffusion at 4°C, AS-B solution is adjusted to 6.5 mg/ml and contains 10 mM MgCl2, 5 mM glutamine, and 10 mM AMP, 0.01 ml of protein solution are mixed with 0.01 ml precipitant solution containing 14% polyethylene glycol 8000 and 50 mM HEPES, pH 7.0, these droplets are suspended against wells containing 15% polyethylene glycol 8000 and 50 mM HEPES, pH 7.0, crystals diffract to 2.0 A

Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
ATP + L-aspartate + L-glutamine	Escherichia coli	-	AMP + diphosphate + L-asparagine + L-glutamate	-	?
ATP + L-aspartate + NH3	Escherichia coli	-	AMP + diphosphate + L-asparagine	-	?
L-glutamine	Escherichia coli	-	L-glutamate + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P22106	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + L-aspartate + L-glutamine	-	Escherichia coli	AMP + diphosphate + L-asparagine + L-glutamate	-	?
ATP + L-aspartate + NH3	-	Escherichia coli	AMP + diphosphate + L-asparagine	-	?
L-glutamine	-	Escherichia coli	L-glutamate + NH3	-	?

Synonyms

Synonyms	Comment	Organism
AS-B	-	Escherichia coli
Asparagine synthetase B	-	Escherichia coli