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Literature summary for 6.3.5.4 extracted from
- Glutamine-dependent asparagine synthetase from Lupinus luteus (1975), Phytochemistry, 14, 1975-1982.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| enzyme requires protection by high levels of thiols, glycerol and substrates also stabilize | Lupinus luteus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Lupinus luteus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
ATP | MgATP2- | Lupinus luteus | |
| 0.16 | - |
Gln | - |
Lupinus luteus | |
| 1.3 | - |
Asp | - |
Lupinus luteus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ cannot be replaced by Zn2+, Fe2+, Co2+, Ba2+ or Ca2+ | Lupinus luteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
substrate-free enzyme, gel filtration | Lupinus luteus |
| 320000 | - |
in presence of MgCl2 and ATP association to a dimer, gel filtration | Lupinus luteus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lupinus luteus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lupinus luteus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Lupinus luteus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Lupinus luteus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C, 60% loss of activity after 3 months | Lupinus luteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Asp + L-Gln | - |
Lupinus luteus | AMP + diphosphate + Asn + Glu | - |
? | |
| additional information | ATP-diphosphate exchange reaction | Lupinus luteus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | dimerization of the 160000 MW enzyme is induced by MgCl2 and ATP | Lupinus luteus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
half-life: 90 s | Lupinus luteus |
| 56 | - |
half-life: 30 s | Lupinus luteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Lupinus luteus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | 8.3 | 80% of maximal activity at pH 6.9 and 8.3 | Lupinus luteus |
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