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Literature summary for 6.3.5.4 extracted from

  • Boehlein, S.K.; Walworth, E.S.; Schuster, S.M.
    Identification of cysteine-523 in the asparatate binding site of Escherichia coli asparagine synthetase B (1997), Biochemistry, 36, 10168-10177.
    View publication on PubMed
Search for more literature for 6.3.5.4

Inhibitors

Inhibitors Comment Organism Structure
5'-O-[p-(fluorosulfonyl)benzoyl]adenosine Cys523 is the key residue involved in the formation of the 5'-O-[p-(fluorosulfonyl)benzoyl]adenosine-induced disulfide bond, inactivation can be reversed by addition of dithiothreitol Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 ATP wild-type Escherichia coli
0.2
-
 ATP ATP, C386A mutant Escherichia coli
0.21
-
 ATP C99A mutant Escherichia coli
0.24
-
 ATP C523A mutant Escherichia coli
0.29
-
 ATP C436A mutant and C514A mutant Escherichia coli
0.37
-
 Gln C523A mutant Escherichia coli
0.39
-
 ATP C168A mutant Escherichia coli
0.44
-
 ATP C423A mutant Escherichia coli
0.51
-
 Gln C386A mutant Escherichia coli
0.68
-
 Asp wild-type enzyme Escherichia coli
0.69
-
 L-Gln wild-type enzyme Escherichia coli
0.74
-
 Gln C436 mutant Escherichia coli
0.77
-
 Gln C423A mutant Escherichia coli
0.83
-
 Gln C168A mutant Escherichia coli
0.85
-
 Asp C436A mutant Escherichia coli
0.88
-
 Asp C386A mutant Escherichia coli
0.92
-
 Gln C514 mutant Escherichia coli
0.96
-
 Asp C99A mutant Escherichia coli
1.68
-
 Asp C168A mutant Escherichia coli
1.7
-
 Asp C514A mutant Escherichia coli
1.91
-
 Asp C423A mutant Escherichia coli
55.7
-
 Asp C523A mutant Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Asp + L-Gln
-
 Escherichia coli AMP + diphosphate + Asn + Glu
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 Gln C523A mutant Escherichia coli
0.52
-
 Asp C523A mutant Escherichia coli
0.53
-
 ATP C523A mutant Escherichia coli
0.69
-
 Gln C386 mutant Escherichia coli
0.73
-
 Gln C436 mutant Escherichia coli
0.74
-
 Gln C514A mutant, Asp, C436A mutant Escherichia coli
0.77
-
 Asp C386A mutant Escherichia coli
0.78
-
 ATP C514A mutant Escherichia coli
0.79
-
 Asp C514A mutant Escherichia coli
0.86
-
 Gln C168 mutant Escherichia coli
0.87
-
 ATP C386A mutant Escherichia coli
1.01
-
 L-Gln wild-type enzyme Escherichia coli
1.05
-
 Asp wild-type enzyme Escherichia coli
1.09
-
 Gln C423A mutant Escherichia coli
1.1
-
 ATP wild-type Escherichia coli
1.15
-
 ATP C168A mutant Escherichia coli
1.17
-
 ATP C99A mutant Escherichia coli
1.23
-
 Asp C168A mutant Escherichia coli
1.31
-
 Gln C99A mutant Escherichia coli
1.35
-
 Asp C423A mutant Escherichia coli
1.37
-
 Asp C99A mutant Escherichia coli
1.46
-
 ATP C423A mutant Escherichia coli