Protein Variants | Comment | Organism |
---|---|---|
N74A | mutant N74A, in which Asn is replaced by Ala can also use NH2OH as an alternative substrate to NH4+ and catalyze the hydrolysis of L-glutamic acid gamma-monohydroxamate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
L-Gln | mutant N74A | Escherichia coli | |
0.09 | - |
L-glutamic acid gamma-monohydroxamate | mutant N74A | Escherichia coli | |
0.26 | - |
L-glutamic acid gamma-monohydroxamate | wild-type enzyme | Escherichia coli | |
0.69 | - |
L-Gln | wild-type enzyme | Escherichia coli | |
5.24 | - |
Gln | mutant C99A | Escherichia coli | |
11.5 | - |
hydroxylamine | wild-type enzyme | Escherichia coli | |
13 | - |
NH4+ | mutant N74A | Escherichia coli | |
15.7 | - |
NH4+ | wild-type enzyme | Escherichia coli | |
17.1 | - |
hydroxylamine | mutant N74A | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and mutant N74A | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Asp + L-Gln | - |
Escherichia coli | AMP + diphosphate + Asn + Glu | - |
? | |
ATP + L-Asp + NH2OH | - |
Escherichia coli | AMP + diphosphate + beta-aspartylhydroxamate | - |
? | |
L-Glutamic acid gamma-monohydroxamate + H2O | - |
Escherichia coli | Hydroxylamine + Glu | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
L-Gln | mutant N74A | Escherichia coli | |
0.1 | - |
L-glutamic acid gamma-monohydroxamate | mutant N74A | Escherichia coli | |
0.15 | - |
L-glutamic acid gamma-monohydroxamate | wild-type | Escherichia coli | |
0.59 | - |
NH4+ | wild-type | Escherichia coli | |
0.65 | - |
NH4+ | mutant N74A | Escherichia coli | |
1.01 | - |
L-Gln | wild-type enzyme | Escherichia coli | |
1.03 | - |
hydroxylamine | wild-type enzyme | Escherichia coli | |
1.17 | - |
hydroxylamine | N74A mutant | Escherichia coli |