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Literature summary for 6.3.5.4 extracted from
- Purification and characterization of beef pancreatic asparagine synthetase (1985), Arch. Biochem. Biophys., 237, 335-346.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | inhibition of the ammonia-dependent reaction, competitive with respect to ammonia, with negative cooperativity. Stimulation of the Gln-dependent and glutaminase reaction | Bos taurus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
Asp | - |
Bos taurus | |
| 0.7 | - |
ATP | - |
Bos taurus | |
| 1.4 | - |
Gln | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | at 16.7 mM CaCl2 31% of the activation relative to MgCl2 | Bos taurus | |
| Cl- | stimulation of the Gln-dependent reaction and glutaminase reaction. Inhibition of the NH4+-dependent reaction, competitive with respect to NH4+, with negative cooperativity | Bos taurus | |
| Co2+ | at 16.7 mM CoCl2 31% of the activation relative to MgCl2 | Bos taurus | |
| FeCl2 | at 16.7 mM FeCl2 39% of the activation relative to MgCl2 | Bos taurus | |
| Mg2+ | 16.7 mM MgCl2, activates | Bos taurus | |
| Mn2+ | at 16.7 mM MnCl2 14% of the activation relative to MgCl2 | Bos taurus | |
| Ni2+ | at 16.7 mM NiCl2 17% of the activation relative to MgCl2 | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, SDS-PAGE | Bos taurus |
| 110000 | 120000 | HPLC gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.17 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Asp + L-Gln | - |
Bos taurus | AMP + diphosphate + Asn + Glu | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Bos taurus |
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Release 2023.1 (January 2023)
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