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Literature summary for 6.3.5.3 extracted from
- The Amidotransferases (1973), Adv. Enzymol. Relat. Areas Mol. Biol., 39, 91-183.
General Stability
| General Stability | Organism |
|---|---|
| K+ required for stabilization | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-diazo-5-oxo-L-norleucine | - |
Gallus gallus |  |
| ADP | - |
Gallus gallus | |
| albizziin | presence of beta-5'-phosphoribosylformylglycinamide, ATP, and Mg2+ inhibits inactivation | Gallus gallus | |
| beta-gamma-5'-Adenylyl methylene diphosphate | - |
Gallus gallus | |
| Cyanate | - |
Gallus gallus | |
| hydroxylamine | - |
Gallus gallus | |
| iodoacetamide | rate of inactivation is 20-30times greater in the presence than in the absence of beta-5'-phosphoribosylformylglycinamide, ATP, and Mg2+ | Gallus gallus | |
| iodoacetate | rate of inactivation is 20-30times greater in the presence than in the absence of beta-5'-phosphoribosylformylglycinamide, ATP, and Mg2+ | Gallus gallus | |
| L-2-Amino-4-oxo-5-chloropentanoic acid | - |
Gallus gallus | |
| Na+ | - |
Gallus gallus | |
| O-diazoacetyl-L-Ser | - |
Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Gallus gallus | |
| additional information | - |
additional information | - |
Mus musculus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can replace Mg2+ in activation | Gallus gallus | |
| K+ | - |
Mus musculus | |
| K+ | enhances the rate of reaction by 20% up to a concentration of 0.1 M, gradual inhibition at higher concentrations | Gallus gallus | |
| Mg2+ | required | Gallus gallus | |
| Mn2+ | can replace Mg2+ in activation | Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 133000 | - |
1 * 133000, SDS-PAGE. When stored in absence of a reducing agent, e.g. DTT or 2-mercaptoethanol, the enzyme undergoes polymerization | Gallus gallus |
| 133000 | 135000 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 133000 | 135000 | - |
Gallus gallus |
| 133000 | 135000 | - |
Pigeon |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-phosphoribosylformylglycinamide + L-Gln | Gallus gallus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
| Mus musculus | - |
- |
- |
| Pigeon | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate | ping-pong mechanism | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
| liver | - |
Gallus gallus | - |
| liver | - |
Pigeon | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-phosphoribosylformylglycinamide + L-Gln | - |
Gallus gallus | ? | - |
? | |
| ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu | - |
? | |
| ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O | - |
Gallus gallus | ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu | - |
? | |
| ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O | - |
Mus musculus | ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu | - |
? | |
| ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O | - |
Pigeon | ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu | - |
? | |
| ATP + 5'-phosphoribosylformylglycinamide + NH4+ + H2O | - |
Gallus gallus | ADP + phosphate + 5'-phosphoribosylformylglycinamidine | - |
? | |
| ATP + 5'-phosphoribosylformylglycinamide + NH4+ + H2O | - |
Mus musculus | ADP + phosphate + 5'-phosphoribosylformylglycinamidine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 133000, SDS-PAGE. When stored in absence of a reducing agent, e.g. DTT or 2-mercaptoethanol, the enzyme undergoes polymerization | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Gallus gallus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
highest stability, rather unstable at pH 8 | Gallus gallus |
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