Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | substrate inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
ATP | at 40°C in 50 mM Tris pH 7.5 | Mycobacterium tuberculosis | |
1.24 | - |
L-glutamine | at 40°C in 50 mM Tris pH 7.5 | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
2 * 56000, SDS-PAGE | Mycobacterium tuberculosis |
137000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + XMP + L-glutamine + H2O | Mycobacterium tuberculosis | - |
AMP + diphosphate + GMP + L-glutamate | - |
? | |
ATP + XMP + L-glutamine + H2O | Mycobacterium tuberculosis H37Rv | - |
AMP + diphosphate + GMP + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, butyl Sepharose column chromatography, and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Storage Stability | Organism |
---|---|
-80°C, in 50 mM Tris pH 7.5, at least 3 months, no loss of activity | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + XMP + L-glutamine + H2O | - |
Mycobacterium tuberculosis | AMP + diphosphate + GMP + L-glutamate | - |
? | |
ATP + XMP + L-glutamine + H2O | - |
Mycobacterium tuberculosis H37Rv | AMP + diphosphate + GMP + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 56000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
GMP synthetase | - |
Mycobacterium tuberculosis |
GMPS | - |
Mycobacterium tuberculosis |
GUA | - |
Mycobacterium tuberculosis |
guanosine monophosphate synthetase | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
XMP | at 40°C in 50 mM Tris pH 7.5 | Mycobacterium tuberculosis | |
2.39 | - |
ATP | at 40°C in 50 mM Tris pH 7.5 | Mycobacterium tuberculosis | |
3.05 | - |
L-glutamine | at 40°C in 50 mM Tris pH 7.5 | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
ATP | at 40°C in 50 mM Tris pH 7.5 | Mycobacterium tuberculosis |