Cloned (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
C102A | totally devoid of glutamine-dependent activity. Kinetic constants for NH4Cl, ATP, and XMP obtained for the ammonia-dependent activity are similar to that of the wild-type enzyme | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.132 | - |
NH3 | pH 7.4, 25°C | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate | evidence for ammonia channeling. Ammonia released from glutamine is not equilibrated with the external medium. Saturating concentrations of glutamine do not obliterate the incorporation of external ammonia into GMP. Ammonia in the external medium can access the thioester intermediate when the ATPPase domain is bound to substrates | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + XMP + L-Gln | enzyme can use both glutamine and external ammonia, the kinetic parameters kcat and Km for the two substrates are different and also exhibit a pH-dependent change | Plasmodium falciparum | AMP + diphosphate + GMP + L-Glu | - |
? | |
ATP + XMP + NH3 | enzyme can use both glutamine and external ammonia, the kinetic parameters kcat and Km for the two substrates are different and also exhibit a pH-dependent change | Plasmodium falciparum | AMP + diphosphate + GMP | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.66 | - |
L-Gln | pH 7.4, 25°C | Plasmodium falciparum | |
0.933 | - |
NH3 | pH 7.4, 25°C | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
glutamine-dependent activity | Plasmodium falciparum |
9.2 | - |
ammonia-dependent activity | Plasmodium falciparum |