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Literature summary for 6.3.5.11 extracted from

  • Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.
    Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans (1990), J. Bacteriol., 172, 6239-6244.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.5.11

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas denitrificans (nom. rej.)

Inhibitors

Inhibitors Comment Organism Structure
(aq)2cobyrinate substrate inhibition above 0.3 mM Pseudomonas denitrificans (nom. rej.)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00021
-
 hydrogenobyrinate c-monoamide pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
0.00041
-
 Hydrogenobyrinate pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
0.0203
-
 L-glutamine pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
0.071
-
 (CN,aq)cobyrinate c-monoamide pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
0.16
-
 (CN,aq)cobyrinate pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
0.25
-
 (aq)2cobyrinate or above, pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
12
-
 ammonia pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 2 * 46000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
86000
-
 gel fitlration Pseudomonas denitrificans (nom. rej.)

Organism

Organism UniProt Comment Textmining
Pseudomonas denitrificans (nom. rej.)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from recombinant Pseudomonas denitrificans Pseudomonas denitrificans (nom. rej.)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1320
-
 pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + (aq)2cobyrinate + 2 L-glutamine + 2 H2O 76% of the initial velocity with cobyrinate Pseudomonas denitrificans (nom. rej.) 2 ADP + 2 phosphate + (aq)2cobyrinate a,c-diamide + 2 L-glutamate
-
 ?
2 ATP + (CN,aq)cobyrinate + 2 L-glutamine + 2 H2O
-
 Pseudomonas denitrificans (nom. rej.) 2 ADP + 2 phosphate + (CN,aq)cobyrinate a,c-diamide + 2 L-glutamate
-
 ?
2 ATP + cobyrinate + 2 ammonia + 2 H2O
-
 Pseudomonas denitrificans (nom. rej.) 2 ADP + 2 phosphate + cobyrinate a,c-diamide
-
 ?
2 ATP + cobyrinate + 2 L-glutamine + 2 H2O
-
 Pseudomonas denitrificans (nom. rej.) 2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate
-
 ?
2 ATP + cobyrinate g-monoamide + L-glutamine + 2 H2O 145% of the initial velocity with cobyrinate Pseudomonas denitrificans (nom. rej.) 2 ADP + phosphate + cobyrinate c,g-diamide + L-glutamate product is 83% cobyrinate c,g-diamide, 17% cobyrinate a,c,g-triamide ?
2 ATP + hydrogenobyrinate + 2 L-glutamine + 2 H2O
-
 Pseudomonas denitrificans (nom. rej.) 2 ADP + 2 phosphate + hydrogenobyrinate a,c-diamide + 2 L-glutamate
-
 ?
ATP + (CN,aq)cobyrinate c-monoamide + L-glutamine + H2O
-
 Pseudomonas denitrificans (nom. rej.) ADP + phosphate + (CN,aq)cobyrinate a,c-diamide + L-glutamate
-
 ?
ATP + cobyrinate a-monoamide + L-glutamine + H2O 81% of the initial velocity with cobyrinate Pseudomonas denitrificans (nom. rej.) ADP + phosphate + cobyrinate a,c-diamide + L-glutamate product is 100% cobyrinate a,c-diamide ?
ATP + cobyrinate c-monoamide + L-glutamine + H2O 19% of the initial velocity with cobyrinate Pseudomonas denitrificans (nom. rej.) ADP + phosphate + cobyrinate a,c-diamide + L-glutamate product is 100% cobyrinate a,c-diamide ?
ATP + hydrogenobyrinate c-monoamide + L-glutamine + H2O 19% of the initial velocity with cobyrinate Pseudomonas denitrificans (nom. rej.) ADP + phosphate + hydrogenobyrinate a,c-diamide + L-glutamate
-
 ?
additional information no further amidation of cobyrinate a,c-diamide and cobyrinate a,c, g-triamide Pseudomonas denitrificans (nom. rej.) ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 46000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 broad Pseudomonas denitrificans (nom. rej.)