Cloned (Comment) | Organism |
---|---|
gene nadE1, sequence comparisons, the nadE1 gene is linked to glnB encoding the signal transduction protein GlnB, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Herbaspirillum seropedicae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten and Hanes-Woolf kinetics, kinetic analysis | Herbaspirillum seropedicae | |
0.01 | - |
deamido-NAD+ | pH 8.5, 30°C, recombinant His-tagged enzyme | Herbaspirillum seropedicae | |
0.031 | - |
ATP | pH 8.5, 30°C, recombinant His-tagged enzyme | Herbaspirillum seropedicae | |
0.365 | - |
L-glutamine | pH 8.5, 30°C, recombinant His-tagged enzyme | Herbaspirillum seropedicae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Herbaspirillum seropedicae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
548000 | - |
recombinant enzyme, gel filtration | Herbaspirillum seropedicae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + L-glutamine + H2O | Herbaspirillum seropedicae | - |
AMP + diphosphate + NAD+ + L-glutamate | - |
? | |
ATP + deamido-NAD+ + L-glutamine + H2O | Herbaspirillum seropedicae SmR1 | - |
AMP + diphosphate + NAD+ + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Herbaspirillum seropedicae | D8IS59 | - |
- |
Herbaspirillum seropedicae SmR1 | D8IS59 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Herbaspirillum seropedicae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + L-glutamine + H2O | - |
Herbaspirillum seropedicae | AMP + diphosphate + NAD+ + L-glutamate | - |
? | |
ATP + deamido-NAD+ + L-glutamine + H2O | - |
Herbaspirillum seropedicae SmR1 | AMP + diphosphate + NAD+ + L-glutamate | - |
? | |
additional information | enzyme NadE1 uses glutamine as a preferential nitrogen donor, poor activity with NH3 | Herbaspirillum seropedicae | ? | - |
? | |
additional information | enzyme NadE1 uses glutamine as a preferential nitrogen donor, poor activity with NH3 | Herbaspirillum seropedicae SmR1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 70000-75000, recombinant enzyme, SDS-PAGE, sequence calculation, and gel filtration | Herbaspirillum seropedicae |
Synonyms | Comment | Organism |
---|---|---|
glutamine-dependent NAD synthetase | - |
Herbaspirillum seropedicae |
glutamine-dependent NadEGln | - |
Herbaspirillum seropedicae |
Hsero_1894 | - |
Herbaspirillum seropedicae |
NadE1 | - |
Herbaspirillum seropedicae |
nicotinamide adenine dinucleotide synthetase enzyme | - |
Herbaspirillum seropedicae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Herbaspirillum seropedicae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Herbaspirillum seropedicae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Herbaspirillum seropedicae |