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Literature summary for 6.3.5.1 extracted from

  • Bellinzoni, M.; Buroni, S.; Pasca, M.R.; Guglierame, P.; Arcesi, F.; De Rossi, E.; Riccardi, G.
    Glutamine amidotransferase activity of NAD+ synthetase from Mycobacterium tuberculosis depends on an amino-terminal nitrilase domain (2005), Res. Microbiol., 156, 173-177.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
C176A complete loss of glutamine-dependent activity, retains 90% of its NH3-dependent activity Mycobacterium tuberculosis
E52A complete loss of glutamine-dependent activity, retains 30% of its NH3-dependent activity Mycobacterium tuberculosis
K121A complete loss of glutamine-dependent and NH3-dependent activity Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
deamido-NAD+ wild-type enzyme Mycobacterium tuberculosis
0.8
-
ATP wild-type enzyme Mycobacterium tuberculosis
0.8
-
ATP mutant enzyme E52A Mycobacterium tuberculosis
1.3
-
ATP mutant enzyme C176A Mycobacterium tuberculosis
1.4
-
deamido-NAD+ mutant enzyme C176A Mycobacterium tuberculosis
1.4
-
deamido-NAD+ mutant enzyme E52A Mycobacterium tuberculosis
1.6
-
L-Gln wild-type enzyme Mycobacterium tuberculosis
1.6
-
NH4+ mutant enzyme E52A Mycobacterium tuberculosis
2.3
-
NH4+ mutant enzyme C176A Mycobacterium tuberculosis
2.7
-
NH4+ wild-type enzyme Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzymes expressed in Escherichia coli Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + L-Gln
-
Mycobacterium tuberculosis AMP + diphosphate + NAD+ + L-Glu
-
?
ATP + deamido-NAD+ + NH4+
-
Mycobacterium tuberculosis AMP + diphosphate + NAD+
-
?

Synonyms

Synonyms Comment Organism
NAD+ synthetase
-
Mycobacterium tuberculosis