Cloned (Comment) | Organism |
---|---|
expression of Nadsyn1 and mutant enzyme C175S as His6-tagged proteins in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C175S | in contrast with the wild-type NADsyn1, the activity of the mutant NADsyn1 (C175S-NADsyn1) is not detected when glutamine is used as a substrate, whereas the activity remains unaltered with NH4Cl | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
deamido-NAD+ | 37°C, wild-type NADsyn1 | Homo sapiens | |
0.089 | - |
ATP | 37°C, wild-type NADsyn1 | Homo sapiens | |
0.12 | - |
ATP | 37°C, NADsyn1 C175S mutant | Homo sapiens | |
0.2 | - |
deamido-NAD+ | 37°C, NADsyn1 C175S mutant | Homo sapiens | |
1.44 | - |
L-glutamine | 37°C, wild-type NADsyn1 | Homo sapiens | |
13.1 | - |
NH3 | 37°C, wild-type NADsyn1 | Homo sapiens | |
23.9 | - |
NH3 | 37°C, NADsyn1 C175S mutant | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
6 * 80000, wild-type and C175S mutant enzyme, SDS-PAGE | Homo sapiens |
80300 | - |
6 * 80300, calculated from sequence | Homo sapiens |
500000 | - |
non-denaturing PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6IA69 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal | Homo sapiens | - |
heart | the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal | Homo sapiens | - |
kidney | the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal | Homo sapiens | - |
liver | the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal | Homo sapiens | - |
lung | the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal | Homo sapiens | - |
skeletal muscle | the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal | Homo sapiens | - |
small intestine | the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal | Homo sapiens | - |
spleen | the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal | Homo sapiens | - |
testis | the major sites of NADsyn1 gene expression are the small intestine, kidney, liver, and testis, whereas the skeletal muscle, spleen, lung, heart, and brain show a weak signal | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + L-glutamine + H2O | NADsyn1 uses not only ammonia but also glutamine. The Vmax/KM-value for NADsyn1 with glutamine is 6.3fold higher than the Vmax/Km-value for NH3 | Homo sapiens | AMP + diphosphate + NAD+ + L-glutamate | - |
? | |
ATP + deamido-NAD+ + NH3 | NADsyn1 uses not only ammonia but also glutamine. The Vmax/KM-value for NADsyn1 with glutamine is 6.3fold higher than the Vmax/Km-value for NH3 | Homo sapiens | AMP + diphosphate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 80000, wild-type and C175S mutant enzyme, SDS-PAGE | Homo sapiens |
hexamer | 6 * 80300, calculated from sequence | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Vmax/KM-value for NADsyn1 with glutamine is 6.3fold higher than the Vmax/Km-value for NH3 | Homo sapiens |