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Literature summary for 6.3.4.9 extracted from

  • Lane, M.D.; Young, D.L.; Lynen, F.
    The enzymatic synthesis of holotranscarboxylase from apotranscarboxylase and (+)-biotin. I. PURIFICATION OF THE APOENZYME AND SYNTHETASE; CHARACTERISTICS OF THE REACTION (1964), J. Biol. Chem., 239, 2858-2864.
    View publication on PubMed
Search for more literature for 6.3.4.9

Activating Compound

Activating Compound Comment Organism Structure
sulfhydryl compounds activate Propionibacterium freudenreichii subsp. shermanii

Inhibitors

Inhibitors Comment Organism Structure
(+)-biotin methyl ester
-
 Propionibacterium freudenreichii subsp. shermanii
(+)-Homobiotin
-
 Propionibacterium freudenreichii subsp. shermanii
DL-O-Heterobiotin
-
 Propionibacterium freudenreichii subsp. shermanii
EDTA
-
 Propionibacterium freudenreichii subsp. shermanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00042
-
 (+)-biotin
-
 Propionibacterium freudenreichii subsp. shermanii
0.002
-
 biotin
-
 Propionibacterium freudenreichii subsp. shermanii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent metal ion required Propionibacterium freudenreichii subsp. shermanii
Mg2+ Mg2+ or Mn2+ Propionibacterium freudenreichii subsp. shermanii
Mn2+ divalent metal ion required, Mg2+ or Mn2+ Propionibacterium freudenreichii subsp. shermanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxyltransferase] Propionibacterium freudenreichii subsp. shermanii
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Propionibacterium freudenreichii subsp. shermanii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Propionibacterium freudenreichii subsp. shermanii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + biocytin + apo-[methylmalonyl-CoA:pyruvate carboxyltransferase]
-
 Propionibacterium freudenreichii subsp. shermanii ?
-
 ?
ATP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxyltransferase]
-
 Propionibacterium freudenreichii subsp. shermanii AMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxyltransferase]
-
 ?
ATP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxyltransferase]
-
 Propionibacterium freudenreichii subsp. shermanii ?
-
 ?
CTP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxyltransferase] 23% of the activity relative to ATP Propionibacterium freudenreichii subsp. shermanii CMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxyltransferase]
-
 ?
GTP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxyltransferase] 18% of the activity relative to ATP Propionibacterium freudenreichii subsp. shermanii GMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxyltransferase]
-
 ?
ITP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxyltransferase] 5% of the activity relative to ATP Propionibacterium freudenreichii subsp. shermanii IMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxyltransferase]
-
 ?
UTP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxyltransferase] 4% of the activity relative to ATP Propionibacterium freudenreichii subsp. shermanii UMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxyltransferase]
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Propionibacterium freudenreichii subsp. shermanii

pH Range

pH Minimum pH Maximum Comment Organism
6 8 6.0: about 45% of maximal activity, 8.0: about 20% of maximal activity Propionibacterium freudenreichii subsp. shermanii