Cloned (Comment) | Organism |
---|---|
- |
Kluyveromyces lactis |
Crystallization (Comment) | Organism |
---|---|
deletion mutant lacking the biotin-carboxyl carrier protein domain, to 6.5 A resolution. Space group P212121, with four monomers per asymmetric unit | Kluyveromyces lactis |
Protein Variants | Comment | Organism |
---|---|---|
G559E/G572E | mutation renders the protein monomeric | Kluyveromyces lactis |
K1605A | mutation in active site of the carboxylyase. A mixture of mutants K1605A and S177A is as efficient as wild-type | Kluyveromyces lactis |
S177A | mutation in the active site of the allophanate hydrolase. A mixture of mutants K1605A and S177A is as efficient as wild-type | Kluyveromyces lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.203 | - |
Urea | mutant S177A, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
0.216 | - |
Urea | mixture of mutants S177A and mutant K1605A, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
0.246 | - |
Urea | isolated urea carboxyxlase domain plus allophanate hydrolase domain, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
0.255 | - |
Urea | wild-type, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
0.299 | - |
Urea | mutant K1605A, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
0.361 | - |
Urea | mutant G559E/G572E, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
500000 | - |
gel filtration | Kluyveromyces lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces lactis | Q6CP22 | enzyme is composed of urea carboxylase domain plus allophanate hydrolase domain, EC 3.5.1.54 | - |
Kluyveromyces lactis DSM 70799 | Q6CP22 | enzyme is composed of urea carboxylase domain plus allophanate hydrolase domain, EC 3.5.1.54 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + urea + HCO3- | - |
Kluyveromyces lactis | ADP + phosphate + urea-1-carboxylate | - |
? | |
ATP + urea + HCO3- | - |
Kluyveromyces lactis DSM 70799 | ADP + phosphate + urea-1-carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 202000, SDS-PAGE | Kluyveromyces lactis |
More | holo-enzyme formation is essential for optimal activity | Kluyveromyces lactis |
Synonyms | Comment | Organism |
---|---|---|
KLLA0_E08119g | - |
Kluyveromyces lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.78 | - |
Urea | mutant S177A, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
1.33 | - |
Urea | mutant K1605A, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
3 | - |
Urea | mutant G559E/G572E, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
10.07 | - |
Urea | isolated urea carboxyxlase domain plus allophanate hydrolase domain, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
12.68 | - |
Urea | mixture of mutants S177A and mutant K1605A, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
13.9 | - |
Urea | wild-type, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.87 | - |
Urea | mutant S177A, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
4.45 | - |
Urea | mutant K1605A, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
8.31 | - |
Urea | mutant G559E/G572E, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
40.9 | - |
Urea | isolated urea carboxyxlase domain plus allophanate hydrolase domain, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
54.6 | - |
Urea | wild-type, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis | |
58.7 | - |
Urea | mixture of mutants S177A and mutant K1605A, pH 8.0, temperature not specified in the publication | Kluyveromyces lactis |